Lihong Fan1, Kunzheng Wang, Bin Cheng. 1. Department of Orthopedics, Second Affiliated Hospital of Xi 'an Jiaotong University, Xi 'an 710004, China.
Abstract
OBJECTIVE: To study the effect of buyang huanwu decoction ([Chinese characters: see text] BYHWD) on spinal ischemia-reperfusion injury and explore the possible mechanism involved. METHOD: 27 model rabbits with ischemia-reperfusion injury were randomly divided into a sham operation group, a model group and a BYHWD-pre-treated group. 24 and 48 hours after the ischemia-reperfusion injury, the motor functions of hind limbs were observed, the expressions of apoptosis-related proteins bcl-2 and Bax in the spinal tissue were investigated with the immunohistochemical methods and apoptosis of nervous cells with TUNEL straining; and the malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in the spinal cord were then determined. RESULTS: BYHWD can improve significantly the motor function of hind limbs, increase SOD activity and decrease the level of MDA, up-regulate expression of Bcl-2 and down-regulated expression of Bax, and depress apoptosis of nervous cells in the spinal cord caused by the ischemia reperfusion in the rabbits. CONCLUSION: BYHWD has a protective action on the spinal ischemia-reperfusion injury, and the mechanisms may be related to the decrease of lipid peroxidation and inhibition of apoptosis of nervous cells.
OBJECTIVE: To study the effect of buyang huanwu decoction ([Chinese characters: see text] BYHWD) on spinal ischemia-reperfusion injury and explore the possible mechanism involved. METHOD: 27 model rabbits with ischemia-reperfusion injury were randomly divided into a sham operation group, a model group and a BYHWD-pre-treated group. 24 and 48 hours after the ischemia-reperfusion injury, the motor functions of hind limbs were observed, the expressions of apoptosis-related proteins bcl-2 and Bax in the spinal tissue were investigated with the immunohistochemical methods and apoptosis of nervous cells with TUNEL straining; and the malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in the spinal cord were then determined. RESULTS: BYHWD can improve significantly the motor function of hind limbs, increase SOD activity and decrease the level of MDA, up-regulate expression of Bcl-2 and down-regulated expression of Bax, and depress apoptosis of nervous cells in the spinal cord caused by the ischemia reperfusion in the rabbits. CONCLUSION: BYHWD has a protective action on the spinal ischemia-reperfusion injury, and the mechanisms may be related to the decrease of lipid peroxidation and inhibition of apoptosis of nervous cells.