Qing Cai1, Zhong-Liang Zhu, Xiao-Li Fan. 1. Department of Physiology and Pathophysiology, Medical School of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
Abstract
AIM: To record calcium and potassium currents in acutely isolated smooth muscle cells of mesenteric arterial branches in rats. METHODS: Smooth muscle cells were freshly isolated by collagenase digest and mechanical trituration with polished pipettes. Patch clamp technique in whole-cell mode was employed to record calcium and potassium currents. RESULTS: The procedure dissociated smooth muscle cells without impairing the electrophysiological characteristics of the cells. The voltage-gated Ca2+ and potassium currents were successfully recorded using whole-cell patch clamp configuration. CONCLUSION: The method dissociates smooth muscle cells from rat mesenteric arterial branches. Voltage-gated channel currents can be recorded in this preparation.
AIM: To record calcium and potassium currents in acutely isolated smooth muscle cells of mesenteric arterial branches in rats. METHODS: Smooth muscle cells were freshly isolated by collagenase digest and mechanical trituration with polished pipettes. Patch clamp technique in whole-cell mode was employed to record calcium and potassium currents. RESULTS: The procedure dissociated smooth muscle cells without impairing the electrophysiological characteristics of the cells. The voltage-gated Ca2+ and potassium currents were successfully recorded using whole-cell patch clamp configuration. CONCLUSION: The method dissociates smooth muscle cells from rat mesenteric arterial branches. Voltage-gated channel currents can be recorded in this preparation.
Authors: Kazi S Jahan; Jian Shi; Harry Z E Greenberg; Sam Khavandi; Miguel Martín-Aragón Baudel; Vincenzo Barrese; Iain A Greenwood; Anthony P Albert Journal: Vascul Pharmacol Date: 2020-07-21 Impact factor: 5.738