Yi Liu1, Rui Wang, Gen-Quan Qiu, Ke-Jun Nan, Xi-Cai Sun. 1. Department of TCM, First Hospital of Xi'an Jiaotong University, Jiankang Road 1#, Xi'an 710061, Shaanxi Province, China. liuyi.jiaotong@163.com
Abstract
AIM: To study the inhibitory effects of fuzheng yiliuyin (Decoction for Suppressing Tumors by Strengthening the Body Resistance) in combination with chemotherapeutics on human gastric carcinoma cell strain. METHODS: Fuzheng yiliuyin (ZY) combined with various kinds of chemotherapeutics was put into two kinds of cultivated human gastric carcinoma cell strains, then its inhibitory effects on human gastric carcinoma cell strains were determined by the MTT method. Flow cytometer was used to assay the apoptosis rate, and the ultrastructure of gastric carcinoma cells was observed under transmission electron microscope. RESULTS: Obvious apoptosis was seen in gastric carcinoma cells after treatment with ZY for 72 h. ZY and chemical drugs had synergistic inhibition effects on the cultivated gastric carcinoma cells, but the effects were different on various cell strains. The inhibitory effects of ZY could be strengthened by cytotoxic action and apoptosis. ZY combined with fluorouracil, etoposide and cisplatin (EFP) chemotherapeutics had better inhibitory effects on SGC-7901, while ZY combined with EFP or with DDP chemotherapeutics had better inhibitory effects than other drugs on MGC-803. CONCLUSION: ZY induces apoptosis and inhibits the growth of gastric carcinoma cells. ZY has the synergistic function of chemotherapeutics.
AIM: To study the inhibitory effects of fuzheng yiliuyin (Decoction for Suppressing Tumors by Strengthening the Body Resistance) in combination with chemotherapeutics on humangastric carcinoma cell strain. METHODS: Fuzheng yiliuyin (ZY) combined with various kinds of chemotherapeutics was put into two kinds of cultivated humangastric carcinoma cell strains, then its inhibitory effects on humangastric carcinoma cell strains were determined by the MTT method. Flow cytometer was used to assay the apoptosis rate, and the ultrastructure of gastric carcinoma cells was observed under transmission electron microscope. RESULTS: Obvious apoptosis was seen in gastric carcinoma cells after treatment with ZY for 72 h. ZY and chemical drugs had synergistic inhibition effects on the cultivated gastric carcinoma cells, but the effects were different on various cell strains. The inhibitory effects of ZY could be strengthened by cytotoxic action and apoptosis. ZY combined with fluorouracil, etoposide and cisplatin (EFP) chemotherapeutics had better inhibitory effects on SGC-7901, while ZY combined with EFP or with DDP chemotherapeutics had better inhibitory effects than other drugs on MGC-803. CONCLUSION: ZY induces apoptosis and inhibits the growth of gastric carcinoma cells. ZY has the synergistic function of chemotherapeutics.
Authors: Joo H Sohn; Hei C Jeung; Hyun J Shin; Sun Y Rha; Jae K Roh; Sung H Noh; Jin S Min; Byung S Kim; Woo I Jang; Hyun C Chung Journal: Am J Clin Oncol Date: 2003-04 Impact factor: 2.339
Authors: Markus Menges; Carsten Schmidt; Werner Lindemann; Karsten Ridwelski; Werner Pueschel; Bernhard Jüngling; Gernot Feifel; Martin Schilling; Andreas Stallmach; Martin Zeitz Journal: J Cancer Res Clin Oncol Date: 2003-06-27 Impact factor: 4.553