Ling Zhang1, Lian Guo, Yong Peng, Bing Chen. 1. Department of Disease Prevention and Health Protection, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
Abstract
AIM: To investigate the effects on telomerase activity of transfection of human T-STAR gene full-length sense cDNA or partial antisense cDNA into human colon cancer cell line HCT-116. METHODS: mRNA and protein expression levels of T-STAR gene were determined by RT-PCR and western blot, and telomerase activity was measured by PCR-ELISA, after transfection of T-STAR sense or antisense gene into HCT-116 cells with lipofectamine. RESULTS: T-STAR gene expression was enhanced or knocked down both at mRNA and protein levels, and telomerase activity was significantly increased or decreased. CONCLUSION: The T-STAR gene may participate in regulation of telomerase activity in human colon cancer HCT-116 cells in a parallel fashion.
AIM: To investigate the effects on telomerase activity of transfection of humanT-STAR gene full-length sense cDNA or partial antisense cDNA into humancolon cancer cell line HCT-116. METHODS: mRNA and protein expression levels of T-STAR gene were determined by RT-PCR and western blot, and telomerase activity was measured by PCR-ELISA, after transfection of T-STAR sense or antisense gene into HCT-116 cells with lipofectamine. RESULTS:T-STAR gene expression was enhanced or knocked down both at mRNA and protein levels, and telomerase activity was significantly increased or decreased. CONCLUSION: The T-STAR gene may participate in regulation of telomerase activity in humancolon cancer HCT-116 cells in a parallel fashion.
Authors: Yuan Lu; Sarra L Ryan; David J Elliott; Graham R Bignell; P Andrew Futreal; David W Ellison; Simon Bailey; Steven C Clifford Journal: PLoS One Date: 2009-07-07 Impact factor: 3.240