Xiao-Peng Tang1, Min Zhang, Xu Yang, Li-Min Chen, Yang Zeng. 1. Research Center of Liver Diseases, the Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China. xiaopeng1959@163.com
Abstract
AIM: To study the condition and potentiality of human umbilical cord blood stem cells (HUCBSC) to differentiate into hepatocytes in vivo or in vitro. METHODS: In a cell culture study of human umbilical cord blood stem cell (HUCBSC) differentiation, human umbilical cord blood mononuclear cells (HUCBMNC) were separated by density gradient centrifugation. Fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) and the supernatant of fetal liver were added in the inducing groups. Only FGF was added in the control group. The expansion and differentiation of HUCBMNC in each group were observed. Human alpha fetoprotein (AFP) and albumin (ALB) were detected by immunohistochemistry. In the animal experiments, the survival SD rats with acute hepatic injury after carbon tetrachloride (CCL4) injection 48 h were randomly divided into three groups. The rats in group A were treated with human umbilical cord blood serum. The rats in group B were treated with HUCBMNC transplantation. The rats in group C were treated with HUCBMNC transplantation followed by intraperitoneal cyclophosphamide for 7 d. The rats were killed at different time points after the treatment and the liver tissue was histopathologically studied and human AFP and ALB detected by immunohistochemistry. The human X inactive-specific transcript gene fragment in the liver tissue was amplified by PCR to find human DNA. RESULTS: The results of cell culture showed that adherent cells were stained negative for AFP or ALB in control group. However, the adherent cells in the inducing groups stained positive for AFP or ALB. The result of animal experiment showed that no human AFP or ALB positive cells present in the liver tissue of group A (control group). However, many human AFP or ALB positive cells were scattered around sinus hepaticus and the central veins of hepatic lobules and in the portal area in group B and group C after one month. The fragment of human X chromagene could be detected in the liver tissue of groups B and C, but not in group A. CONCLUSION: Under certain conditions HUCBSC can differentiate into liver cells in vivo and in vitro.
AIM: To study the condition and potentiality of human umbilical cord blood stem cells (HUCBSC) to differentiate into hepatocytes in vivo or in vitro. METHODS: In a cell culture study of human umbilical cord blood stem cell (HUCBSC) differentiation, human umbilical cord blood mononuclear cells (HUCBMNC) were separated by density gradient centrifugation. Fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) and the supernatant of fetal liver were added in the inducing groups. Only FGF was added in the control group. The expansion and differentiation of HUCBMNC in each group were observed. Human alpha fetoprotein (AFP) and albumin (ALB) were detected by immunohistochemistry. In the animal experiments, the survival SD rats with acute hepatic injury after carbon tetrachloride (CCL4) injection 48 h were randomly divided into three groups. The rats in group A were treated with human umbilical cord blood serum. The rats in group B were treated with HUCBMNC transplantation. The rats in group C were treated with HUCBMNC transplantation followed by intraperitoneal cyclophosphamide for 7 d. The rats were killed at different time points after the treatment and the liver tissue was histopathologically studied and humanAFP and ALB detected by immunohistochemistry. The human X inactive-specific transcript gene fragment in the liver tissue was amplified by PCR to find human DNA. RESULTS: The results of cell culture showed that adherent cells were stained negative for AFP or ALB in control group. However, the adherent cells in the inducing groups stained positive for AFP or ALB. The result of animal experiment showed that no humanAFP or ALB positive cells present in the liver tissue of group A (control group). However, many humanAFP or ALB positive cells were scattered around sinus hepaticus and the central veins of hepatic lobules and in the portal area in group B and group C after one month. The fragment of human X chromagene could be detected in the liver tissue of groups B and C, but not in group A. CONCLUSION: Under certain conditions HUCBSC can differentiate into liver cells in vivo and in vitro.
Authors: E Lagasse; H Connors; M Al-Dhalimy; M Reitsma; M Dohse; L Osborne; X Wang; M Finegold; I L Weissman; M Grompe Journal: Nat Med Date: 2000-11 Impact factor: 53.440
Authors: M R Alison; R Poulsom; R Jeffery; A P Dhillon; A Quaglia; J Jacob; M Novelli; G Prentice; J Williamson; N A Wright Journal: Nature Date: 2000-07-20 Impact factor: 49.962
Authors: I Avital; D Inderbitzin; T Aoki; D B Tyan; A H Cohen; C Ferraresso; J Rozga; W S Arnaout; A A Demetriou Journal: Biochem Biophys Res Commun Date: 2001-10-19 Impact factor: 3.575
Authors: V Rocha; J Cornish; E L Sievers; A Filipovich; F Locatelli; C Peters; M Remberger; G Michel; W Arcese; S Dallorso; K Tiedemann; A Busca; K W Chan; S Kato; J Ortega; M Vowels; A Zander; G Souillet; A Oakill; A Woolfrey; A L Pay; A Green; F Garnier; I Ionescu; P Wernet; G Sirchia; P Rubinstein; S Chevret; E Gluckman Journal: Blood Date: 2001-05-15 Impact factor: 22.113
Authors: V Rocha; J E Wagner; K A Sobocinski; J P Klein; M J Zhang; M M Horowitz; E Gluckman Journal: N Engl J Med Date: 2000-06-22 Impact factor: 91.245
Authors: Philip N Newsome; Ingolfur Johannessen; Shelagh Boyle; Evangelos Dalakas; Karen A McAulay; Kay Samuel; Frances Rae; Lesley Forrester; Marc L Turner; Peter C Hayes; David J Harrison; Wendy A Bickmore; John N Plevris Journal: Gastroenterology Date: 2003-06 Impact factor: 22.682
Authors: Alaa Ismail; Ehsan Hassan; Mohamed I Seleem; Medhat Hassan; Firas Z ElDeen; Ahmed Salah; Abdulhafez A Selim Journal: Int J Stem Cells Date: 2010-05 Impact factor: 2.500
Authors: Eman A Toraih; Manal S Fawzy; Abdullah I El-Falouji; Elham O Hamed; Nader A Nemr; Mohammad H Hussein; Noha M Abd El Fadeal Journal: Mol Med Date: 2016-09-12 Impact factor: 6.354