| Literature DB >> 1681074 |
Y S Taktak1, S Selkirk, A F Bristow, A Carpenter, C Ball, B Rafferty, S Poole.
Abstract
A novel in-vitro system has been developed for the detection and quantification of pyrogen in pharmaceutical products. The measured variable was evoked secretion of the pyrogenic cytokine interleukin-6 from MONO MAC 6 monocytic cells incubated with the product. The interleukin-6 was detected using a specific and sensitive ELISA developed for this purpose. The test system detected pyrogenic contamination in 3 batches of therapeutic human serum albumin which had caused adverse reactions in recipients. The contamination was not detected in conventional tests: the rabbit pyrogen test and the limulus amoebocyte lysate test.Entities:
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Year: 1991 PMID： 1681074 DOI： 10.1111/j.2042-7158.1991.tb03540.x
Source DB: PubMed Journal: J Pharm Pharmacol ISSN： 0022-3573 Impact factor: 3.765