CONCLUSION: Triamcinolone acetonide crystalline suspension (e.g. Volon A) was not ototoxic to the auditory hair cells present within organ of Corti explants and protected them from an ototoxic molecule, i.e. 4-hydroxy-2,3-nonenal (HNE), that is produced within the organ of Corti as a result of oxidative stress-induced damage. OBJECTIVES: To test the corticosteroid, triamcinolone acetonide, for ototoxicity and otoprotective capacity in organ of Corti explants. MATERIALS AND METHODS: Organ of Corti explants excised from 4-day-old rats were the test system, HNE was the ototoxin challenge. Hair cell integrity counts were performed with fluorescent microscopy on fixed explants stained with FITC-labeled phalloidin. Statistical significance was set at p<0.05. RESULTS: Triamcinolone acetonide did not affect hair cell integrity in the organ of Corti explants and it provided a high level of protection of hair cells against the ototoxic effects of a damaging level of HNE as determined by hair cell density counts.
CONCLUSION:Triamcinolone acetonide crystalline suspension (e.g. Volon A) was not ototoxic to the auditory hair cells present within organ of Corti explants and protected them from an ototoxic molecule, i.e. 4-hydroxy-2,3-nonenal (HNE), that is produced within the organ of Corti as a result of oxidative stress-induced damage. OBJECTIVES: To test the corticosteroid, triamcinolone acetonide, for ototoxicity and otoprotective capacity in organ of Corti explants. MATERIALS AND METHODS: Organ of Corti explants excised from 4-day-old rats were the test system, HNE was the ototoxin challenge. Hair cell integrity counts were performed with fluorescent microscopy on fixed explants stained with FITC-labeled phalloidin. Statistical significance was set at p<0.05. RESULTS:Triamcinolone acetonide did not affect hair cell integrity in the organ of Corti explants and it provided a high level of protection of hair cells against the ototoxic effects of a damaging level of HNE as determined by hair cell density counts.
Authors: Xiaoping Du; Wei Li; Xinsheng Gao; Matthew B West; W Mark Saltzman; Christopher J Cheng; Charles Stewart; Jie Zheng; Weihua Cheng; Richard D Kopke Journal: Hear Res Date: 2013-07-09 Impact factor: 3.208
Authors: Elisabeth Engleder; Clemens Honeder; Julia Klobasa; Michael Wirth; Christoph Arnoldner; Franz Gabor Journal: Int J Pharm Date: 2014-06-02 Impact factor: 5.875
Authors: Clemens Honeder; Elisabeth Engleder; Hanna Schöpper; Franz Gabor; Gottfried Reznicek; Jens Wagenblast; Wolfgang Gstoettner; Christoph Arnoldner Journal: Audiol Neurootol Date: 2014-04-02 Impact factor: 1.854