BACKGROUND: It has been known that transcription factor nuclear factor (NF)-kappaB plays an important role in cell proliferation and oncogenesis. The aims of this study were to evaluate expression levels of NF-kappaB in nonsmall-cell lung cancer (NSCLC) and to elucidate its clinical significance and prognostic value for patients with NSCLC. METHODS: Using 45 tumor tissue specimens from 45 patients with NSCLC who underwent surgery, we investigated the expression of NF-kappaB using Western blotting analysis. Apoptotic rate of NSCLC cells with different expression of NF-kappaB was determined by TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling) assay. Paraffin-embedded tissue blocks from 71 consecutive patients with NSCLC were obtained for immunohistochemical staining. RESULTS: The expression level of NF-kappaB in poorly or moderately differentiated lung cancer cells was higher than that in well-differentiated ones (p = 0.001). The apoptotic rate was lower for lung cancer cells with higher NF-kappaB expression than for those with lower NF-kappaB expression (p = 0.0238). Furthermore, expression of NF-kappaB was correlated with caspase-3, cyclooxygenase-2, and p53 expression in lung cancer cells that were examined. Most NSCLC cells showed nuclear staining pattern and the nuclear positive rate was 67.6% (48 of 71 specimens). Immunohistochemical NF-kappaB expression in patients with NSCLC was an independent prognostic factor for overall survival. CONCLUSIONS: The results of this study suggest that expression of NF-kappaB may correlate with lung cancer differentiation. Overexpression of NF-kappaB inhibits tumor cell apoptosis and indicates an unfavorable prognosis for overall survival in some patients with NSCLC.
BACKGROUND: It has been known that transcription factor nuclear factor (NF)-kappaB plays an important role in cell proliferation and oncogenesis. The aims of this study were to evaluate expression levels of NF-kappaB in nonsmall-cell lung cancer (NSCLC) and to elucidate its clinical significance and prognostic value for patients with NSCLC. METHODS: Using 45 tumor tissue specimens from 45 patients with NSCLC who underwent surgery, we investigated the expression of NF-kappaB using Western blotting analysis. Apoptotic rate of NSCLC cells with different expression of NF-kappaB was determined by TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling) assay. Paraffin-embedded tissue blocks from 71 consecutive patients with NSCLC were obtained for immunohistochemical staining. RESULTS: The expression level of NF-kappaB in poorly or moderately differentiated lung cancer cells was higher than that in well-differentiated ones (p = 0.001). The apoptotic rate was lower for lung cancer cells with higher NF-kappaB expression than for those with lower NF-kappaB expression (p = 0.0238). Furthermore, expression of NF-kappaB was correlated with caspase-3, cyclooxygenase-2, and p53 expression in lung cancer cells that were examined. Most NSCLC cells showed nuclear staining pattern and the nuclear positive rate was 67.6% (48 of 71 specimens). Immunohistochemical NF-kappaB expression in patients with NSCLC was an independent prognostic factor for overall survival. CONCLUSIONS: The results of this study suggest that expression of NF-kappaB may correlate with lung cancer differentiation. Overexpression of NF-kappaB inhibits tumor cell apoptosis and indicates an unfavorable prognosis for overall survival in some patients with NSCLC.
Authors: Jamie A Saxon; Taylor P Sherrill; Vasiliy V Polosukhin; Jiqing Sai; Rinat Zaynagetdinov; Allyson G McLoed; Peter M Gulleman; Whitney Barham; Dong-Sheng Cheng; Raphael P Hunt; Linda A Gleaves; Ann Richmond; Lisa R Young; Fiona E Yull; Timothy S Blackwell Journal: Oncoimmunology Date: 2016-03-30 Impact factor: 8.110
Authors: C E Badr; J M Niers; D Morse; J A Koelen; P Vandertop; D Noske; T Wurdinger; P A Zalloua; B A Tannous Journal: Gene Ther Date: 2010-12-09 Impact factor: 5.250
Authors: Christian E Badr; Johanna M Niers; Lee-Ann Tjon-Kon-Fat; David P Noske; Thomas Wurdinger; Bakhos A Tannous Journal: Mol Imaging Date: 2009 Sep-Oct Impact factor: 4.488
Authors: Fotinos-Ioannis D Dimitrakopoulos; Anna G Antonacopoulou; Anastasia Kottorou; Helen Vlotinou; Nikolaos D Panagopoulos; Dimitrios Dougenis; Chrisoula Scopa; Helen Papadaki; Haralabos P Kalofonos Journal: Virchows Arch Date: 2012-05-05 Impact factor: 4.064
Authors: Michael R Mehan; Deborah Ayers; Derek Thirstrup; Wei Xiong; Rachel M Ostroff; Edward N Brody; Jeffrey J Walker; Larry Gold; Thale C Jarvis; Nebojsa Janjic; Geoffrey S Baird; Sheri K Wilcox Journal: PLoS One Date: 2012-04-11 Impact factor: 3.240