| Literature DB >> 16797237 |
Tomoko Watanabe1, Jun-ichi Masuyama, Yoshiaki Sohma, Hiroko Inazawa, Kaori Horie, Kumiko Kojima, Yasunori Uemura, Yumi Aoki, Shuji Kaga, Seiji Minota, Toshiyuki Tanaka, Yasunori Yamaguchi, Tetsuto Kobayashi, Isao Serizawa.
Abstract
We previously reported that 4C8 monoclonal antibody (mAb) provides a costimulatory signal to human CD4+ T cells and consequently induces regulatory T (Treg) cells, which are hypo-responsive and suppress the polyclonal response of bystander CD4+ cells in a contact-dependent manner. In this study, we identified the antigen of 4C8 mAb as CD52. Costimulation with Campath-1H, a humanized anti-CD52 mAb, also induced Treg cells. Anti-CD52-induced Treg cells suppressed the proliferation of both CD4+ and CD8+ T cells provided with polyclonal or allogeneic stimulation. When Treg cells were induced from Staphylococcal enterotoxin B (SEB) treated cells, they suppressed the response to SEB more efficiently than that to another superantigen, SEA. Furthermore, anti-CD52-induced Treg cells could be expanded by culture with IL-2 followed by CD52-costimulation, and co-injection of expanded Treg cells suppressed lethal xenogeneic graft versus host disease (GvHD) reactions in SCID mice caused by human peripheral blood mononuclear cells (PBMCs).Entities:
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Year: 2006 PMID： 16797237 DOI： 10.1016/j.clim.2006.05.006
Source DB: PubMed Journal: Clin Immunol ISSN： 1521-6616 Impact factor: 3.969