| Literature DB >> 16797201 |
Jukka Sirén1, Tadaatsu Imaizumi, Devanand Sarkar, Taija Pietilä, Diana L Noah, Rongtuan Lin, John Hiscott, Robert M Krug, Paul B Fisher, Ilkka Julkunen, Sampsa Matikainen.
Abstract
Activation of host cell antiviral responses is mediated by pattern recognition receptors. Cytoplasmic RNA helicases, retinoic acid inducible gene-I (RIG-I) and melanoma differentiation-associated gene 5 (mda-5) have been identified to function as receptors for double-stranded RNA. Here we show that interferon (IFN)-alpha pretreatment enhances influenza A virus-induced expression of IFN-alpha, IFN-beta, interleukin (IL)-28 and IL-29 genes in human dendritic cells and epithelial cell lines. Both IFN-alpha and IFN-beta strongly enhanced RIG-I and mda-5 mRNA and protein expression in these cell types. Expression of RIG-I and mda-5 gene constructs, but not that of TLR3, lead to a dramatic enhancement of IFN-beta promoter driven transcription in influenza A virus-infected epithelial cells. Furthermore, dominant negative RIG-I gene construct inhibited influenza A virus-induced IFN-beta promoter activity. In conclusion, our results show that in epithelial cells influenza A virus-induced antiviral cytokine gene expression is triggered by RIG-I and mda-5, whose expression is positively regulated by IFN-alpha.Entities:
Mesh：
Substances：
Year: 2006 PMID： 16797201 DOI： 10.1016/j.micinf.2006.02.028
Source DB: PubMed Journal: Microbes Infect ISSN： 1286-4579 Impact factor: 2.700