| Literature DB >> 16794773 |
R Capparelli1, D Palumbo, M Iannaccone, I Ventimiglia, E Di Salle, F Capuano, P Salvatore, M G Amoroso.
Abstract
Antimicrobial peptides and proteins are being studied with increasing interest because of their broad range antimicrobial activity. Among plant antimicrobial proteins, the wheat seed polypeptides, puroindoline a and puroindoline b, are particularly interesting because of their established antibacterial activity. In this paper we describe different strategies used to clone His tagged and GST tagged puroindolines obtaining 1.5 mg recombinant protein from 1 l culture. The antimicrobial activity of recombinant and native puroindolines was comparable.Entities:
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Year: 2006 PMID： 16794773 DOI： 10.1007/s10529-006-9031-9
Source DB: PubMed Journal: Biotechnol Lett ISSN： 0141-5492 Impact factor: 2.461