Literature DB >> 16794711

Multidimensional single-molecule imaging in live cells using total-internal-reflection fluorescence microscopy.

S E D Webb1, S R Needham, S K Roberts, M L Martin-Fernandez.   

Abstract

We have developed a wide-field total-internal-reflection fluorescence microscope capable of imaging single molecules in live cells, resolved in both wavelength and polarization. We show fluorescence resonance energy transfer between single pairs of fluorescent molecules bound to signaling receptors in the plasma membrane of live cells and demonstrate the importance of polarization discrimination in addition to wavelength separation.

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Year:  2006        PMID： 16794711     DOI： 10.1364/ol.31.002157

Source DB:  PubMed          Journal:  Opt Lett        ISSN： 0146-9592            Impact factor:   3.776


  13 in total

1.  A hybrid model for erythrocyte membrane: a single unit of protein network coupled with lipid bilayer.

Authors:  Qiang Zhu; Carlos Vera; Robert J Asaro; Paul Sche; L Amy Sung
Journal:  Biophys J       Date:  2007-04-20       Impact factor: 4.033

2.  Kinetics of complexin binding to the SNARE complex: correcting single molecule FRET measurements for hidden events.

Authors:  Yulong Li; George J Augustine; Keith Weninger
Journal:  Biophys J       Date:  2007-05-18       Impact factor: 4.033

3.  Two-dimensional standing wave total internal reflection fluorescence microscopy: superresolution imaging of single molecular and biological specimens.

Authors:  Euiheon Chung; Daekeun Kim; Yan Cui; Yang-Hyo Kim; Peter T C So
Journal:  Biophys J       Date:  2007-05-04       Impact factor: 4.033

4.  Single-molecule spectroscopy and imaging of biomolecules in living cells.

Authors:  Samuel J Lord; Hsiao-lu D Lee; W E Moerner
Journal:  Anal Chem       Date:  2010-03-15       Impact factor: 6.986

5.  Automated multidimensional single molecule fluorescence microscopy feature detection and tracking.

Authors:  Daniel J Rolfe; Charles I McLachlan; Michael Hirsch; Sarah R Needham; Christopher J Tynan; Stephen E D Webb; Marisa L Martin-Fernandez; Michael P Hobson
Journal:  Eur Biophys J       Date:  2011-09-18       Impact factor: 1.733

6.  Subcellular and single-molecule imaging of plant fluorescent proteins using total internal reflection fluorescence microscopy (TIRFM).

Authors:  Gema Vizcay-Barrena; Stephen E D Webb; Marisa L Martin-Fernandez; Zoe A Wilson
Journal:  J Exp Bot       Date:  2011-08-23       Impact factor: 6.992

7.  Visualization of BRI1 and SERK3/BAK1 Nanoclusters in Arabidopsis Roots.

Authors:  Stefan J Hutten; Danny S Hamers; Marije Aan den Toorn; Wilma van Esse; Antsje Nolles; Christoph A Bücherl; Sacco C de Vries; Johannes Hohlbein; Jan Willem Borst
Journal:  PLoS One       Date:  2017-01-23       Impact factor: 3.240

8.  Single-molecule imaging and fluorescence lifetime imaging microscopy show different structures for high- and low-affinity epidermal growth factor receptors in A431 cells.

Authors:  Stephen E D Webb; Selene K Roberts; Sarah R Needham; Christopher J Tynan; Daniel J Rolfe; Martyn D Winn; David T Clarke; Roger Barraclough; Marisa L Martin-Fernandez
Journal:  Biophys J       Date:  2007-09-21       Impact factor: 4.033

Review 9.  Single molecule fluorescence detection and tracking in mammalian cells: the state-of-the-art and future perspectives.

Authors:  Marisa L Martin-Fernandez; David T Clarke
Journal:  Int J Mol Sci       Date:  2012-11-13       Impact factor: 5.923

10.  The architecture of EGFR's basal complexes reveals autoinhibition mechanisms in dimers and oligomers.

Authors:  Laura C Zanetti-Domingues; Dimitrios Korovesis; Sarah R Needham; Christopher J Tynan; Shiori Sagawa; Selene K Roberts; Antonija Kuzmanic; Elena Ortiz-Zapater; Purvi Jain; Rob C Roovers; Alireza Lajevardipour; Paul M P van Bergen En Henegouwen; George Santis; Andrew H A Clayton; David T Clarke; Francesco L Gervasio; Yibing Shan; David E Shaw; Daniel J Rolfe; Peter J Parker; Marisa L Martin-Fernandez
Journal:  Nat Commun       Date:  2018-10-18       Impact factor: 14.919
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