Literature DB >> 16793391

Purification and biochemical characterization of ataxia-telangiectasia mutated and Mre11/Rad50/Nbs1.

Ji-Hoon Lee1, Tanya T Paull.   

Abstract

Ataxia-telangiectasia mutated (ATM) is a serine-threonine kinase that is activated by DNA double strand breaks to phosphorylate many cellular proteins involved in cell cycle regulation and DNA repair. We have shown previously that the activation of ATM can be reconstituted in an in vitro system using recombinant human ATM. In this system, ATM activity is dependent on the Mre11/Rad50/Nbs1 (MRN) complex and linear DNA, similar to requirements observed in human cells. This chapter describes methods used for the overexpression and purification of human ATM and MRN, as well as a protocol for in vitro kinase assays.

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Year:  2006        PMID： 16793391     DOI： 10.1016/S0076-6879(06)08033-5

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  27 in total

1.  Biochemical characterization of bacteriophage T4 Mre11-Rad50 complex.

Authors:  Timothy J Herdendorf; Dustin W Albrecht; Stephen J Benkovic; Scott W Nelson
Journal:  J Biol Chem       Date:  2010-11-15       Impact factor: 5.157

2.  ATM directs DNA damage responses and proteostasis via genetically separable pathways.

Authors:  Ji-Hoon Lee; Michael R Mand; Chung-Hsuan Kao; Yi Zhou; Seung W Ryu; Alicia L Richards; Joshua J Coon; Tanya T Paull
Journal:  Sci Signal       Date:  2018-01-09       Impact factor: 8.192

3.  Visualization of local DNA unwinding by Mre11/Rad50/Nbs1 using single-molecule FRET.

Authors:  Brian Cannon; Jeffrey Kuhnlein; Soo-Hyun Yang; Anita Cheng; Detlev Schindler; Jeremy M Stark; Rick Russell; Tanya T Paull
Journal:  Proc Natl Acad Sci U S A       Date:  2013-11-04       Impact factor: 11.205

4.  MRE11-RAD50-NBS1 is a critical regulator of FANCD2 stability and function during DNA double-strand break repair.

Authors:  Céline Roques; Yan Coulombe; Mathieu Delannoy; Julien Vignard; Simona Grossi; Isabelle Brodeur; Amélie Rodrigue; Jean Gautier; Alicja Z Stasiak; Andrzej Stasiak; Angelos Constantinou; Jean-Yves Masson
Journal:  EMBO J       Date:  2009-07-16       Impact factor: 11.598

5.  An interaction between the Walker A and D-loop motifs is critical to ATP hydrolysis and cooperativity in bacteriophage T4 Rad50.

Authors:  Metzere Bierlein De la Rosa; Scott W Nelson
Journal:  J Biol Chem       Date:  2011-05-24       Impact factor: 5.157

6.  The DNA damage response kinases DNA-dependent protein kinase (DNA-PK) and ataxia telangiectasia mutated (ATM) Are stimulated by bulky adduct-containing DNA.

Authors:  Michael G Kemp; Laura A Lindsey-Boltz; Aziz Sancar
Journal:  J Biol Chem       Date:  2011-04-12       Impact factor: 5.157

7.  53BP1 promotes ATM activity through direct interactions with the MRN complex.

Authors:  Ji-Hoon Lee; Aaron A Goodarzi; Penny A Jeggo; Tanya T Paull
Journal:  EMBO J       Date:  2009-12-10       Impact factor: 11.598

8.  Ancient and recent adaptive evolution of primate non-homologous end joining genes.

Authors:  Ann Demogines; Alysia M East; Ji-Hoon Lee; Sharon R Grossman; Pardis C Sabeti; Tanya T Paull; Sara L Sawyer
Journal:  PLoS Genet       Date:  2010-10-21       Impact factor: 5.917

9.  PIKK-dependent phosphorylation of Mre11 induces MRN complex inactivation by disassembly from chromatin.

Authors:  Michela Di Virgilio; Carol Y Ying; Jean Gautier
Journal:  DNA Repair (Amst)       Date:  2009-08-25

10.  RAD50 and NBS1 form a stable complex functional in DNA binding and tethering.

Authors:  Eddy van der Linden; Humberto Sanchez; Eri Kinoshita; Roland Kanaar; Claire Wyman
Journal:  Nucleic Acids Res       Date:  2009-01-16       Impact factor: 16.971
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