PURPOSE: The aim of this study was to determine the effect of hyperinsulinemia on myocardial and hepatic distribution and metabolism of 14(R,S)-[18F]fluoro-6-thia-heptadecanoic acid ([18F]FTHA). PROCEDURES: Mitochondrial retention and intracellular lipid incorporation of [18F]FTHA were compared to that of [14C]-2-bromopalmitate or [14C]palmitate during hyperinsulinemic clamp vs. saline infusion in male Wistar rats. RESULTS: Mitochondrial 18F activity was increased in the heart (1.7 +/- 0.4 vs. 0.5 +/- 0.1% ID/g, P < 0.05), whereas it was reduced in the liver (1.1 +/- 0.3 vs. 1.8 +/- 0.4% ID/g, P < 0.05) during insulin vs. saline infusion, respectively. Mitochondrial [14C]-2-bromopalmitate activity was affected by insulin in a similar way in both tissues. The fractional esterification of [18F]FTHA into triglycerides was impaired compared to [14C]palmitate in both tissues, and [18F]FTHA was insensitive to the shift of esterification of fatty acids into complex lipids in response to insulin. CONCLUSIONS: [18F]FTHA is sensitive to insulin-induced modifications of free fatty acid oxidative metabolism in rats but is insensitive to changes in nonoxidative fatty acid metabolism.
PURPOSE: The aim of this study was to determine the effect of hyperinsulinemia on myocardial and hepatic distribution and metabolism of 14(R,S)-[18F]fluoro-6-thia-heptadecanoic acid ([18F]FTHA). PROCEDURES: Mitochondrial retention and intracellular lipid incorporation of [18F]FTHA were compared to that of [14C]-2-bromopalmitate or [14C]palmitate during hyperinsulinemic clamp vs. saline infusion in male Wistar rats. RESULTS: Mitochondrial 18F activity was increased in the heart (1.7 +/- 0.4 vs. 0.5 +/- 0.1% ID/g, P < 0.05), whereas it was reduced in the liver (1.1 +/- 0.3 vs. 1.8 +/- 0.4% ID/g, P < 0.05) during insulin vs. saline infusion, respectively. Mitochondrial [14C]-2-bromopalmitate activity was affected by insulin in a similar way in both tissues. The fractional esterification of [18F]FTHA into triglycerides was impaired compared to [14C]palmitate in both tissues, and [18F]FTHA was insensitive to the shift of esterification of fatty acids into complex lipids in response to insulin. CONCLUSIONS: [18F]FTHA is sensitive to insulin-induced modifications of free fatty acid oxidative metabolism in rats but is insensitive to changes in nonoxidative fatty acid metabolism.
Authors: J P Felber; E Ferrannini; A Golay; H U Meyer; D Theibaud; B Curchod; E Maeder; E Jequier; R A DeFronzo Journal: Diabetes Date: 1987-11 Impact factor: 9.461
Authors: Teemu O Takala; Pirjo Nuutila; Kari Pulkki; Vesa Oikonen; Tove Grönroos; Timo Savunen; Tommi Vähäsilta; Matti Luotolahti; Markku Kallajoki; Jörgen Bergman; Sarita Forsback; Juhani Knuuti Journal: Eur J Nucl Med Mol Imaging Date: 2002-10-03 Impact factor: 9.236
Authors: Patricia Iozzo; Anu K Turpeinen; Teemu Takala; Vesa Oikonen; Olof Solin; Ele Ferrannini; Pirjo Nuutila; Juhani Knuuti Journal: Eur J Nucl Med Mol Imaging Date: 2003-06-17 Impact factor: 9.236
Authors: Véronique Ouellet; Sébastien M Labbé; Denis P Blondin; Serge Phoenix; Brigitte Guérin; François Haman; Eric E Turcotte; Denis Richard; André C Carpentier Journal: J Clin Invest Date: 2012-01-24 Impact factor: 14.808
Authors: Fang Zhang; Guiyang Hao; Mengle Shao; Kien Nham; Yu An; Qiong Wang; Yi Zhu; Christine M Kusminski; Gedaa Hassan; Rana K Gupta; Qiwei Zhai; Xiankai Sun; Philipp E Scherer; Orhan K Oz Journal: Cell Metab Date: 2018-01-09 Impact factor: 27.287
Authors: Sébastien L Ménard; Xiuli Ci; Frédérique Frisch; François Normand-Lauzière; Jules Cadorette; René Ouellet; Johannes E Van Lier; François Bénard; M'hamed Bentourkia; Roger Lecomte; André C Carpentier Journal: Mol Imaging Biol Date: 2008-09-04 Impact factor: 3.488
Authors: Sébastien M Labbé; Etienne Croteau; Thomas Grenier-Larouche; Frédérique Frisch; René Ouellet; Réjean Langlois; Brigitte Guérin; Eric E Turcotte; André C Carpentier Journal: Diabetes Date: 2011-01-12 Impact factor: 9.461
Authors: Sébastien M Labbé; Thomas Grenier-Larouche; Christophe Noll; Serge Phoenix; Brigitte Guérin; Eric E Turcotte; André C Carpentier Journal: Diabetes Date: 2012-11 Impact factor: 9.461