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Literature DB >> 16791212

Overview: methods and applications for droplet compartmentalization of biology.

John H Leamon¹, Darren R Link, Michael Egholm, Jonathan M Rothberg.

Abstract

Three protocols in this issue highlight applications of emulsification procedures, which deliver high-throughput potential to the molecular biology laboratory, without the need for automation. These procedures have already generated interesting results and spurred the development of exciting new technologies, while requiring only readily available laboratory equipment.

Mesh：

Substances：
Emulsions

Year: 2006 PMID： 16791212 DOI： 10.1038/nmeth0706-541

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

Keyword Cloud
Cited

17 in total

1. Monitoring single-cell bioenergetics via the coarsening of emulsion droplets.

Authors: L Boitard; D Cottinet; C Kleinschmitt; N Bremond; J Baudry; G Yvert; J Bibette
Journal: Proc Natl Acad Sci U S A Date: 2012-04-25 Impact factor: 11.205

2. Comparison of the Illumina Genome Analyzer and Roche 454 GS FLX for resequencing of hypertrophic cardiomyopathy-associated genes.

Authors: Shale Dames; Jacob Durtschi; Katherine Geiersbach; Jack Stephens; Karl V Voelkerding
Journal: J Biomol Tech Date: 2010-07

3. Piezoelectric-driven droplet impact printing with an interchangeable microfluidic cartridge.

Authors: Baoqing Li; Jinzhen Fan; Jiannan Li; Jiaru Chu; Tingrui Pan
Journal: Biomicrofluidics Date: 2015-09-01 Impact factor: 2.800

4. Hybrid capture and next-generation sequencing identify viral integration sites from formalin-fixed, paraffin-embedded tissue.

Authors: Eric J Duncavage; Vincent Magrini; Nils Becker; Jon R Armstrong; Ryan T Demeter; Todd Wylie; Haley J Abel; John D Pfeifer
Journal: J Mol Diagn Date: 2011-05 Impact factor: 5.568

Overview: methods and applications for droplet compartmentalization of biology.

1. Monitoring single-cell bioenergetics via the coarsening of emulsion droplets.

2. Comparison of the Illumina Genome Analyzer and Roche 454 GS FLX for resequencing of hypertrophic cardiomyopathy-associated genes.

3. Piezoelectric-driven droplet impact printing with an interchangeable microfluidic cartridge.

4. Hybrid capture and next-generation sequencing identify viral integration sites from formalin-fixed, paraffin-embedded tissue.

5. Short homologous sequences are strongly associated with the generation of chimeric RNAs in eukaryotes.

6. A highly parallel microfluidic droplet method enabling single-molecule counting for digital enzyme detection.

7. Chemistry with spatial control using particles and streams().

8. Targeted sequencing of the human X chromosome exome.

9. Development of an in vitro compartmentalization screen for high-throughput directed evolution of [FeFe] hydrogenases.

10. High-throughput quantitative polymerase chain reaction in picoliter droplets.