BACKGROUND/AIMS: Cell adhesion molecules play a critical role in the invasion and metastasis of a variety of human tumors. Abnormal expression of VCAM-1 has been demonstrated to correlate with the malignant progression of gastric tumors, but the molecular mechanism underlying the VCAM-1-dependent metastasis has been rarely investigated. To explore the role for tumor cell-expressing adhesion molecules in the carcinoma-endothelium adhesion, we analyzed expression status of adhesion molecules in gastric cancer cells and its association with tumor cell capability of endothelial adhesion. METHODS: Endothelial adhesion ability of gastric tumor cells was tested using calcein AM staining assay. Expression of cell surface proteins was determined by Western blot, flow cytometry, and immunofluorescence assays. RNAi-mediated knockdown of gene expression and neutralization with specific antibodies were utilized for functional analysis. RESULTS: One of three cell lines tested was identified to be adhesive to endothelial cells and express VCAM-1. Adherence ability of the cells was dramatically decreased by neutralization of surface VCAM-1. VCAM-1 was co-localized with Caveolin-1 and siRNA-mediated knockdown of Caveolin-1 expression significantly blocked the VCAM-1-dependent cell adhesion. CONCLUSIONS: Our data imply important roles for VCAM-1 and Caveolin- 1 in the regulation of metastatic potential of gastric tumor cells.
BACKGROUND/AIMS: Cell adhesion molecules play a critical role in the invasion and metastasis of a variety of humantumors. Abnormal expression of VCAM-1 has been demonstrated to correlate with the malignant progression of gastric tumors, but the molecular mechanism underlying the VCAM-1-dependent metastasis has been rarely investigated. To explore the role for tumor cell-expressing adhesion molecules in the carcinoma-endothelium adhesion, we analyzed expression status of adhesion molecules in gastric cancer cells and its association with tumor cell capability of endothelial adhesion. METHODS: Endothelial adhesion ability of gastric tumor cells was tested using calcein AM staining assay. Expression of cell surface proteins was determined by Western blot, flow cytometry, and immunofluorescence assays. RNAi-mediated knockdown of gene expression and neutralization with specific antibodies were utilized for functional analysis. RESULTS: One of three cell lines tested was identified to be adhesive to endothelial cells and express VCAM-1. Adherence ability of the cells was dramatically decreased by neutralization of surface VCAM-1. VCAM-1 was co-localized with Caveolin-1 and siRNA-mediated knockdown of Caveolin-1 expression significantly blocked the VCAM-1-dependent cell adhesion. CONCLUSIONS: Our data imply important roles for VCAM-1 and Caveolin- 1 in the regulation of metastatic potential of gastric tumor cells.
Authors: John H Chidlow; John D Glawe; J Steven Alexander; Christopher G Kevil Journal: Am J Physiol Gastrointest Liver Physiol Date: 2010-09-30 Impact factor: 4.052
Authors: Yean Jung Choi; Xabier Arzuaga; Chase T Kluemper; Adelka Caraballo; Michal Toborek; Bernhard Hennig Journal: Environ Int Date: 2009-07-15 Impact factor: 9.621
Authors: Magdalena Król; Karol M Pawłowski; Katarzyna Szyszko; Henryk Maciejewski; Izabella Dolka; Elisabetta Manuali; Michał Jank; Tomasz Motyl Journal: BMC Vet Res Date: 2012-03-27 Impact factor: 2.741
Authors: Stefan Riwaldt; Johann Bauer; Jessica Pietsch; Markus Braun; Jürgen Segerer; Achim Schwarzwälder; Thomas J Corydon; Manfred Infanger; Daniela Grimm Journal: Int J Mol Sci Date: 2015-11-30 Impact factor: 5.923