| Literature DB >> 167878 |
M H Cohen, D J Drage, A Robertson.
Abstract
The design, calibration, and operation of a source of controlled amounts of cyclic AMP (c-AMP) are described. Typically, 1.5 s pulses containing 10(10)-10(-12) molecules of c-AMP can be delivered to a region about 10 mum in diameter on an agar plate. The resulting concentration profiles are given as functions of distance and time. The diffusion coefficient of c-AMP in agar was measured to be 0.97 times 10(-5) cm2-s-1 at 21 degrees C.Entities:
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Year: 1975 PMID: 167878 PMCID: PMC1334778 DOI: 10.1016/S0006-3495(75)85852-8
Source DB: PubMed Journal: Biophys J ISSN: 0006-3495 Impact factor: 4.033