Literature DB >> 16785648

Proteomic analysis by multidimensional protein identification technology.

Laurence Florens1, Michael P Washburn.   

Abstract

Multidimensional chromatography coupled to mass spectrometry is an emerging technique for the analysis of complex protein mixtures. One approach in this general category, multidimensional protein identification technology (MudPIT), couples biphasic or triphasic microcapillary columns to high-performance liquid chromatography, tandem mass spectrometry, and database searching. The integration of each of these components is critical to the implementation of MudPIT in a laboratory. MudPIT can be used for the analysis of complex peptide mixtures generated from biofluids, tissues, cells, organelles, or protein complexes. The information described in this chapter will provide researchers with details for sample preparation, column assembly, and chromatography parameters for complex peptide mixture analysis.

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Year:  2006        PMID： 16785648     DOI： 10.1385/1-59745-026-X:159

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  118 in total

1.  Ssu72 phosphatase-dependent erasure of phospho-Ser7 marks on the RNA polymerase II C-terminal domain is essential for viability and transcription termination.

Authors:  David W Zhang; Amber L Mosley; Sreenivasa R Ramisetty; Juan B Rodríguez-Molina; Michael P Washburn; Aseem Z Ansari
Journal:  J Biol Chem       Date:  2012-01-10       Impact factor: 5.157

2.  Characterization of a highly conserved histone related protein, Ydl156w, and its functional associations using quantitative proteomic analyses.

Authors:  Joshua M Gilmore; Mihaela E Sardiu; Swaminathan Venkatesh; Brent Stutzman; Allison Peak; Chris W Seidel; Jerry L Workman; Laurence Florens; Michael P Washburn
Journal:  Mol Cell Proteomics       Date:  2011-12-22       Impact factor: 5.911

3.  Relative, label-free protein quantitation: spectral counting error statistics from nine replicate MudPIT samples.

Authors:  Bret Cooper; Jian Feng; Wesley M Garrett
Journal:  J Am Soc Mass Spectrom       Date:  2010-05-06       Impact factor: 3.109

4.  A Role for Widely Interspaced Zinc Finger (WIZ) in Retention of the G9a Methyltransferase on Chromatin.

Authors:  Jeremy M Simon; Joel S Parker; Feng Liu; Scott B Rothbart; Slimane Ait-Si-Ali; Brian D Strahl; Jian Jin; Ian J Davis; Amber L Mosley; Samantha G Pattenden
Journal:  J Biol Chem       Date:  2015-09-03       Impact factor: 5.157

Review 5.  Analyzing chromatin remodeling complexes using shotgun proteomics and normalized spectral abundance factors.

Authors:  Laurence Florens; Michael J Carozza; Selene K Swanson; Marjorie Fournier; Michael K Coleman; Jerry L Workman; Michael P Washburn
Journal:  Methods       Date:  2006-12       Impact factor: 3.608

6.  Lentiviral Vpr usurps Cul4-DDB1[VprBP] E3 ubiquitin ligase to modulate cell cycle.

Authors:  Kasia Hrecka; Magdalena Gierszewska; Smita Srivastava; Lukasz Kozaczkiewicz; Selene K Swanson; Laurence Florens; Michael P Washburn; Jacek Skowronski
Journal:  Proc Natl Acad Sci U S A       Date:  2007-07-03       Impact factor: 11.205

7.  Genomic and proteomic analysis of phiEco32, a novel Escherichia coli bacteriophage.

Authors:  Dhruti Savalia; Lars F Westblade; Manisha Goel; Laurence Florens; Priscilla Kemp; Natalja Akulenko; Olga Pavlova; Julio C Padovan; Brian T Chait; Michael P Washburn; Hans-W Ackermann; Arcady Mushegian; Tarasii Gabisonia; Ian Molineux; Konstantin Severinov
Journal:  J Mol Biol       Date:  2008-01-11       Impact factor: 5.469

8.  Genome comparison and proteomic characterization of Thermus thermophilus bacteriophages P23-45 and P74-26: siphoviruses with triplex-forming sequences and the longest known tails.

Authors:  Leonid Minakhin; Manisha Goel; Zhanna Berdygulova; Erlan Ramanculov; Laurence Florens; Galina Glazko; Valeri N Karamychev; Alexei I Slesarev; Sergei A Kozyavkin; Igor Khromov; Hans-W Ackermann; Michael Washburn; Arcady Mushegian; Konstantin Severinov
Journal:  J Mol Biol       Date:  2008-02-15       Impact factor: 5.469

9.  A novel histone fold domain-containing protein that replaces TAF6 in Drosophila SAGA is required for SAGA-dependent gene expression.

Authors:  Vikki M Weake; Selene K Swanson; Arcady Mushegian; Laurence Florens; Michael P Washburn; Susan M Abmayr; Jerry L Workman
Journal:  Genes Dev       Date:  2009-12-15       Impact factor: 11.361

10.  Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex.

Authors:  Yong Cai; Jingji Jin; Selene K Swanson; Michael D Cole; Seung Hyuk Choi; Laurence Florens; Michael P Washburn; Joan W Conaway; Ronald C Conaway
Journal:  J Biol Chem       Date:  2009-12-14       Impact factor: 5.157
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