OBJECTIVE: The significance of negative sentinel lymph nodes (SLN) is important in the staging and treatment of melanoma and a few other cancers, but is controversial in uterine cervix carcinoma. Our study was aimed at correlating the SLN status in cervical carcinoma with non-sentinel lymph nodes (non-SLN), in a uniform and well controlled population. METHODS: This study includes 36 patients with stage I and IIA cervical carcinoma and bilaterally negative SLN on final pathology. SLN were identified using blue dye and radioisotopic techniques. Frozen section examination was performed for all SLN; the rest of the tissue was formalin fixed and paraffin embedded. The protocol used for SLN was also applied for non-SLN. For each block, six 4-micro m thick sections were cut at 40 micro m intervals and stained with H and E; an additional section taken between the 3rd and 4th levels was imunostained using AE1/AE3 cytokeratin. RESULTS: The mean age for the study population was 39 years (range 25-76); the number of SLN ranged from 2 to 6 (mean 2.7) and the non-SLN from 8 to 49 (mean 23) per case. No metastasis was found in any SLN and non-SLN by step sections and IHC. CONCLUSION: Our study demonstrates that bilaterally negative SLN on final pathology accurately predict the absence of metastases in non-SLN in cervical carcinoma. If confirmed by larger trials, these results may influence the clinical management of early cervical cancer.
OBJECTIVE: The significance of negative sentinel lymph nodes (SLN) is important in the staging and treatment of melanoma and a few other cancers, but is controversial in uterine cervix carcinoma. Our study was aimed at correlating the SLN status in cervical carcinoma with non-sentinel lymph nodes (non-SLN), in a uniform and well controlled population. METHODS: This study includes 36 patients with stage I and IIA cervical carcinoma and bilaterally negative SLN on final pathology. SLN were identified using blue dye and radioisotopic techniques. Frozen section examination was performed for all SLN; the rest of the tissue was formalin fixed and paraffin embedded. The protocol used for SLN was also applied for non-SLN. For each block, six 4-micro m thick sections were cut at 40 micro m intervals and stained with H and E; an additional section taken between the 3rd and 4th levels was imunostained using AE1/AE3 cytokeratin. RESULTS: The mean age for the study population was 39 years (range 25-76); the number of SLN ranged from 2 to 6 (mean 2.7) and the non-SLN from 8 to 49 (mean 23) per case. No metastasis was found in any SLN and non-SLN by step sections and IHC. CONCLUSION: Our study demonstrates that bilaterally negative SLN on final pathology accurately predict the absence of metastases in non-SLN in cervical carcinoma. If confirmed by larger trials, these results may influence the clinical management of early cervical cancer.
Authors: Tarik Z Belhocine; Michel Prefontaine; Dominique Lanvin; Monique Bertrand; Irina Rachinsky; Helen Ettler; Pamela Zabel; Larry W Stitt; Akira Sugimoto; Jean-Luc Urbain Journal: Am J Nucl Med Mol Imaging Date: 2013-03-08
Authors: Kristyna Nemejcova; Roman Kocian; Christhardt Kohler; Jiri Jarkovsky; Jaroslav Klat; Alberto Berjon; Radovan Pilka; Borek Sehnal; Blanca Gil-Ibanez; Ezequiel Lupo; Almerinda Petiz; Octavio Arencibia Sanchez; Peter Kascak; Fabio Martinelli; Alessandro Buda; Jiri Presl; Marc Barahona; Luc van Lonkhuijzen; Wiktor Szatkowski; Lubos Minar; Maja Pakiz; Pavel Havelka; Cristina Zorrero; Marcin Misiek; Leon Cornelius Snyman; Dariusz Wydra; Ignace Vergote; Alla Vinnytska; Mikulas Redecha; Martin Michal; Solveig Tingulstad; Barbara Kipp; Grzegorz Szewczyk; Robert Toth; Francisco Javier de Santiago Garcia; Pluvio Jesus Coronado Martin; Robert Poka; Karl Tamussino; Mathieu Luyckx; Maxime Fastrez; Juan Carlos Staringer; Anna Germanova; Andrea Plaikner; Sylva Bajsova; Pavel Dundr; Nina Mallmann-Gottschalk; David Cibula Journal: Cancers (Basel) Date: 2020-04-29 Impact factor: 6.639