Literature DB >> 16780196

In situ hybridization of whole-mount embryos.

Murray Hargrave1, Josephine Bowles, Peter Koopman.   

Abstract

The ability to visualize the expression of a gene in both time and space is an essential tool of developmental biology. Here, we detail a robust method for in situ hybridization of RNA probes to whole pieces of fixed tissue. This method has been optimized for reliable and sensitive visualization of the spatial patterns of gene expression in mouse embryo tissue.

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Year:  2006        PMID： 16780196     DOI： 10.1385/1-59745-007-3:103

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  17 in total

1.  Uncovering gene regulatory networks during mouse fetal germ cell development.

Authors:  Antoine D Rolland; Kim P Lehmann; Kamin J Johnson; Kevin W Gaido; Peter Koopman
Journal:  Biol Reprod       Date:  2010-12-08       Impact factor: 4.285

2.  Transcriptomic analysis of avian digits reveals conserved and derived digit identities in birds.

Authors:  Zhe Wang; Rebecca L Young; Huiling Xue; Günter P Wagner
Journal:  Nature       Date:  2011-09-04       Impact factor: 49.962

3.  Expression and functional analysis of Dkk1 during early gonadal development.

Authors:  A N Combes; J Bowles; C-W Feng; H S Chiu; P-L Khoo; A Jackson; M H Little; P P L Tam; P Koopman
Journal:  Sex Dev       Date:  2011-05-24       Impact factor: 1.824

4.  Transcription factors ER71/ETV2 and SOX9 participate in a positive feedback loop in fetal and adult mouse testis.

Authors:  Luciano DiTacchio; Josephine Bowles; Sook Shin; Dae-Sik Lim; Peter Koopman; Ralf Janknecht
Journal:  J Biol Chem       Date:  2012-05-21       Impact factor: 5.157

5.  The cerebellin 4 precursor gene is a direct target of SRY and SOX9 in mice.

Authors:  Stephen T Bradford; Ryuji Hiramatsu; Madhavi P Maddugoda; Pascal Bernard; Marie-Christine Chaboissier; Andrew Sinclair; Andreas Schedl; Vincent Harley; Yoshiakira Kanai; Peter Koopman; Dagmar Wilhelm
Journal:  Biol Reprod       Date:  2009-02-11       Impact factor: 4.285

6.  Functional characterization of tissue-specific enhancers in the DLX5/6 locus.

Authors:  Ramon Y Birnbaum; David B Everman; Karl K Murphy; Fiorella Gurrieri; Charles E Schwartz; Nadav Ahituv
Journal:  Hum Mol Genet       Date:  2012-08-21       Impact factor: 6.150

7.  Three-dimensional visualization of testis cord morphogenesis, a novel tubulogenic mechanism in development.

Authors:  Alexander N Combes; Emmanuelle Lesieur; Vincent R Harley; Andrew H Sinclair; Melissa H Little; Dagmar Wilhelm; Peter Koopman
Journal:  Dev Dyn       Date:  2009-05       Impact factor: 3.780

8.  Unraveling the transcriptional regulation of TWIST1 in limb development.

Authors:  Naama Hirsch; Reut Eshel; Reut Bar Yaacov; Tal Shahar; Fania Shmulevich; Idit Dahan; Noam Levaot; Tommy Kaplan; Darío G Lupiáñez; Ramon Y Birnbaum
Journal:  PLoS Genet       Date:  2018-10-29       Impact factor: 5.917

9.  Coding exons function as tissue-specific enhancers of nearby genes.

Authors:  Ramon Y Birnbaum; E Josephine Clowney; Orly Agamy; Mee J Kim; Jingjing Zhao; Takayuki Yamanaka; Zachary Pappalardo; Shoa L Clarke; Aaron M Wenger; Loan Nguyen; Fiorella Gurrieri; David B Everman; Charles E Schwartz; Ohad S Birk; Gill Bejerano; Stavros Lomvardas; Nadav Ahituv
Journal:  Genome Res       Date:  2012-03-22       Impact factor: 9.043

10.  Deletion of ultraconserved elements yields viable mice.

Authors:  Nadav Ahituv; Yiwen Zhu; Axel Visel; Amy Holt; Veena Afzal; Len A Pennacchio; Edward M Rubin
Journal:  PLoS Biol       Date:  2007-09       Impact factor: 8.029
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