OBJECTIVE: To study the constituents with the pain-relieving activity from the stem rind of Daphne giraldii. METHOD: The partition of the ethanol extract and chromatographic separation of the fractions were carried out by the monitoring of anelgesic pharmacological activity. The structures of the isolated compounds were determined by MS and NMR. RESULT: Four compounds were isolated from the pain-relieving fraction. Three of them were identified as diterpenes, gniditrin (1), gnidicin (2) and daphnetoxin (3). Compound 4 was determined as Z-octadecyl caffeate. CONCLUSION: Compounds 1, 2 and 4 were isolated from the plant for the first time.
OBJECTIVE: To study the constituents with the pain-relieving activity from the stem rind of Daphne giraldii. METHOD: The partition of the ethanol extract and chromatographic separation of the fractions were carried out by the monitoring of anelgesic pharmacological activity. The structures of the isolated compounds were determined by MS and NMR. RESULT: Four compounds were isolated from the pain-relieving fraction. Three of them were identified as diterpenes, gniditrin (1), gnidicin (2) and daphnetoxin (3). Compound 4 was determined as Z-octadecyl caffeate. CONCLUSION: Compounds 1, 2 and 4 were isolated from the plant for the first time.