Literature DB >> 16775356

Structure-based mutational analysis of the NS3 helicase from dengue virus.

Aruna Sampath1, Ting Xu, Alex Chao, Dahai Luo, Julien Lescar, Subhash G Vasudevan.   

Abstract

We performed a mutational analysis of the NS3 helicase of dengue virus to test insights gleaned from its crystal structure and identified four residues in the full-length protein that severely impaired either its RTPase and ATPase (Arg-457-458, Arg-460, Arg-463) or helicase (Ile-365, Arg-376) activity. Alanine substitution of Lys-396, which is located at the surface of domain II, drastically reduced all three enzymatic activities. Our study points to a pocket at the surface of domain II that may be suitable for the design of allosteric inhibitors.

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Year:  2006        PMID： 16775356      PMCID： PMC1488930          DOI： 10.1128/JVI.02215-05

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  23 in total

1.  A small region of the dengue virus-encoded RNA-dependent RNA polymerase, NS5, confers interaction with both the nuclear transport receptor importin-beta and the viral helicase, NS3.

Authors:  Magnus Johansson; Andrew J Brooks; David A Jans; Subhash G Vasudevan
Journal:  J Gen Virol       Date:  2001-04       Impact factor: 3.891

2.  Mutagenesis of the Dengue virus type 2 NS3 protein within and outside helicase motifs: effects on enzyme activity and virus replication.

Authors:  A E Matusan; M J Pryor; A D Davidson; P J Wright
Journal:  J Virol       Date:  2001-10       Impact factor: 5.103

3.  The functional interaction of the hepatitis C virus helicase molecules is responsible for unwinding processivity.

Authors:  Mikhail K Levin; Yuh-Hwa Wang; Smita S Patel
Journal:  J Biol Chem       Date:  2004-04-14       Impact factor: 5.157

4.  RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP.

Authors:  Sophie Dumont; Wei Cheng; Victor Serebrov; Rudolf K Beran; Ignacio Tinoco; Anna Marie Pyle; Carlos Bustamante
Journal:  Nature       Date:  2006-01-05       Impact factor: 49.962

5.  Purification and characterization of West Nile virus nucleoside triphosphatase (NTPase)/helicase: evidence for dissociation of the NTPase and helicase activities of the enzyme.

Authors:  P Borowski; A Niebuhr; O Mueller; M Bretner; K Felczak; T Kulikowski; H Schmitz
Journal:  J Virol       Date:  2001-04       Impact factor: 5.103

6.  Identification and characterization of the RNA helicase activity of Japanese encephalitis virus NS3 protein.

Authors:  A Utama; H Shimizu; S Morikawa; F Hasebe; K Morita; A Igarashi; M Hatsu; K Takamizawa; T Miyamura
Journal:  FEBS Lett       Date:  2000-01-07       Impact factor: 4.124

7.  ATP-binding domain of NTPase/helicase as a target for hepatitis C antiviral therapy.

Authors:  P Borowski; O Mueller; A Niebuhr; M Kalitzky; L H Hwang; H Schmitz; M A Siwecka; T Kulikowsk
Journal:  Acta Biochim Pol       Date:  2000       Impact factor: 2.149

8.  Expression, purification, and characterization of the RNA 5'-triphosphatase activity of dengue virus type 2 nonstructural protein 3.

Authors:  Greg Bartelma; R Padmanabhan
Journal:  Virology       Date:  2002-07-20       Impact factor: 3.616

9.  The RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities of Dengue virus protein NS3 are Mg2+-dependent and require a functional Walker B motif in the helicase catalytic core.

Authors:  Delphine Benarroch; Barbara Selisko; Giada A Locatelli; Giovanni Maga; Jean-Louis Romette; Bruno Canard
Journal:  Virology       Date:  2004-10-25       Impact factor: 3.616

10.  Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes.

Authors:  A E Gorbalenya; E V Koonin; A P Donchenko; V M Blinov
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971
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  28 in total

1.  Structural and functional parameters of the flaviviral protease: a promising antiviral drug target.

Authors:  Sergey A Shiryaev; Alex Y Strongin
Journal:  Future Virol       Date:  2010-09-01       Impact factor: 1.831

2.  Comparative analysis of full-length genomic sequences of 10 dengue serotype 1 viruses associated with different genotypes, epidemics, and disease severity isolated in Thailand over 22 years.

Authors:  Yuxin Tang; Prinyada Rodpradit; Piyawan Chinnawirotpisan; Mammen P Mammen; Tao Li; Julia A Lynch; Robert Putnak; Chunlin Zhang
Journal:  Am J Trop Med Hyg       Date:  2010-11       Impact factor: 2.345

3.  Crystal structure of the dengue virus RNA-dependent RNA polymerase catalytic domain at 1.85-angstrom resolution.

Authors:  Thai Leong Yap; Ting Xu; Yen-Liang Chen; Helene Malet; Marie-Pierre Egloff; Bruno Canard; Subhash G Vasudevan; Julien Lescar
Journal:  J Virol       Date:  2007-02-14       Impact factor: 5.103

4.  Crystal structure of the NS3 protease-helicase from dengue virus.

Authors:  Dahai Luo; Ting Xu; Cornelia Hunke; Gerhard Grüber; Subhash G Vasudevan; Julien Lescar
Journal:  J Virol       Date:  2007-10-17       Impact factor: 5.103

Review 5.  Molecular targets for flavivirus drug discovery.

Authors:  Aruna Sampath; R Padmanabhan
Journal:  Antiviral Res       Date:  2008-09-15       Impact factor: 5.970

6.  NS3 helicase from dengue virus specifically recognizes viral RNA sequence to ensure optimal replication.

Authors:  Crystall M D Swarbrick; Chandrakala Basavannacharya; Kitti W K Chan; Shu-Ann Chan; Daljit Singh; Na Wei; Wint Wint Phoo; Dahai Luo; Julien Lescar; Subhash G Vasudevan
Journal:  Nucleic Acids Res       Date:  2017-12-15       Impact factor: 16.971

7.  Motif V regulates energy transduction between the flavivirus NS3 ATPase and RNA-binding cleft.

Authors:  Kelly E Du Pont; Russell B Davidson; Martin McCullagh; Brian J Geiss
Journal:  J Biol Chem       Date:  2019-12-30       Impact factor: 5.157

Review 8.  Organization of the Flavivirus RNA replicase complex.

Authors:  Carolin Brand; Martin Bisaillon; Brian J Geiss
Journal:  Wiley Interdiscip Rev RNA       Date:  2017-08-16       Impact factor: 9.957

9.  The C-terminal 50 amino acid residues of dengue NS3 protein are important for NS3-NS5 interaction and viral replication.

Authors:  Moon Y F Tay; Wuan Geok Saw; Yongqian Zhao; Kitti W K Chan; Daljit Singh; Yuwen Chong; Jade K Forwood; Eng Eong Ooi; Gerhard Grüber; Julien Lescar; Dahai Luo; Subhash G Vasudevan
Journal:  J Biol Chem       Date:  2014-12-08       Impact factor: 5.157

10.  Design, synthesis and characterization of novel 1,2-benzisothiazol-3(2H)-one and 1,3,4-oxadiazole hybrid derivatives: potent inhibitors of Dengue and West Nile virus NS2B/NS3 proteases.

Authors:  Huiguo Lai; Dengfeng Dou; Sridhar Aravapalli; Tadahisa Teramoto; Gerald H Lushington; Tom M Mwania; Kevin R Alliston; David M Eichhorn; Radhakrishnan Padmanabhan; William C Groutas
Journal:  Bioorg Med Chem       Date:  2012-11-15       Impact factor: 3.641
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