Literature DB >> 16770740

Staurosporine-induced growth inhibition of glioma cells is accompanied by altered expression of cyclins, CDKs and CDK inhibitors.

Mugdha N Harmalkar1, Neelam V Shirsat.   

Abstract

Staurosporine was found to bring about complete growth inhibition of human glioma cell lines. U87 MG cells were arrested in S phase while U373 MG cells in G2/M phase on staurosporine treatment. Consistent with this observation, no change in G1 phase regulators viz., Cyclin D1, D3 and CDK4 was seen on staurosporine treatment. The levels of CDK2, CDC2, Cyclin A and Cyclin B proteins decreased, while the levels of CDK inhibitors viz., p21 and p27 were found to increase on staurosporine treatment. The mRNA levels of CDK2 and CDC2 genes were also found to decrease on staurosporine treatment. Thus apart from staurosporine's known direct inhibitory effect on CDK2 and CDC2 activities, staurosporine was found to down-regulate activities of these two kinases by modulating the expression of the kinases themselves as well that of their activating partners (Cyclins) and their inhibitors.

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 16770740     DOI： 10.1007/s11064-006-9068-0

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  22 in total

1.  Different effects of staurosporine, an inhibitor of protein kinases, on the cell cycle and chromatin structure of normal and leukemic lymphocytes.

Authors:  S Bruno; B Ardelt; J S Skierski; F Traganos; Z Darzynkiewicz
Journal:  Cancer Res       Date:  1992-01-15       Impact factor: 12.701

2.  Sodium butyrate sensitizes human glioma cells to TRAIL-mediated apoptosis through inhibition of Cdc2 and the subsequent downregulation of survivin and XIAP.

Authors:  Eun Hee Kim; Hee Sue Kim; Seung U Kim; Eun Joo Noh; Jong-Soo Lee; Kyeong Sook Choi
Journal:  Oncogene       Date:  2005-10-20       Impact factor: 9.867

3.  Overexpression of protein kinase C epsilon in astroglial brain tumor derived cell lines and primary tumor samples.

Authors:  T R Sharif; M Sharif
Journal:  Int J Oncol       Date:  1999-08       Impact factor: 5.650

4.  Regulation of apoptosis at cell division by p34cdc2 phosphorylation of survivin.

Authors:  D S O'Connor; D Grossman; J Plescia; F Li; H Zhang; A Villa; S Tognin; P C Marchisio; D C Altieri
Journal:  Proc Natl Acad Sci U S A       Date:  2000-11-21       Impact factor: 11.205

5.  Protein kinase C in cultured adult human oligodendrocytes: a potential role for isoform alpha as a mediator of process outgrowth.

Authors:  V W Yong; N P Dooley; P G Noble
Journal:  J Neurosci Res       Date:  1994-09-01       Impact factor: 4.164

6.  Staurosporine-induced apoptosis is independent of p16 and p21 and achieved via arrest at G2/M and at G1 in U251MG human glioma cell line.

Authors:  Fumiyuki Yamasaki; Seiji Hama; Hiroyuki Yoshioka; Yoshinori Kajiwara; Kaita Yahara; Kazuhiko Sugiyama; Yuji Heike; Kazunori Arita; Kaoru Kurisu
Journal:  Cancer Chemother Pharmacol       Date:  2003-03-20       Impact factor: 3.333

7.  Protein kinase C activity correlates with the growth rate of malignant gliomas: Part II. Effects of glioma mitogens and modulators of protein kinase C.

Authors:  W T Couldwell; J P Antel; V W Yong
Journal:  Neurosurgery       Date:  1992-10       Impact factor: 4.654

8.  Different susceptibility of protein kinases to staurosporine inhibition. Kinetic studies and molecular bases for the resistance of protein kinase CK2.

Authors:  F Meggio; A Donella Deana; M Ruzzene; A M Brunati; L Cesaro; B Guerra; T Meyer; H Mett; D Fabbro; P Furet
Journal:  Eur J Biochem       Date:  1995-11-15

9.  The kinase inhibitor staurosporine induces G1 arrest at two points: effect on retinoblastoma protein phosphorylation and cyclin-dependent kinase 2 in normal and transformed cells.

Authors:  J B Schnier; D M Gadbois; K Nishi; E M Bradbury
Journal:  Cancer Res       Date:  1994-11-15       Impact factor: 12.701

10.  Control of apoptosis and mitotic spindle checkpoint by survivin.

Authors:  F Li; G Ambrosini; E Y Chu; J Plescia; S Tognin; P C Marchisio; D C Altieri
Journal:  Nature       Date:  1998-12-10       Impact factor: 49.962
View more
  1 in total

1.  Lanatoside C sensitizes glioblastoma cells to tumor necrosis factor-related apoptosis-inducing ligand and induces an alternative cell death pathway.

Authors:  Christian E Badr; Thomas Wurdinger; Jonas Nilsson; Johanna M Niers; Michael Whalen; Alexei Degterev; Bakhos A Tannous
Journal:  Neuro Oncol       Date:  2011-07-13       Impact factor: 12.300
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.