| Literature DB >> 1676912 |
Abstract
Polymerase chain reaction (PCR) amplification of the second exon of DRB genes from genomic DNA is described. Sequence-specific oligonucleotide (SSO) probes have been designed to allow for the typing of DRB alleles. The methods are described in detail and the results of a trial on both homozygous typing cells and restriction fragment length polymorphism (RFLP)-typed local caucasoid controls are given. The ease of use of this form of DRB typing is emphasized and potential complications are discussed.Entities:
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Year: 1991 PMID: 1676912 DOI: 10.1111/j.1744-313x.1991.tb00007.x
Source DB: PubMed Journal: Eur J Immunogenet ISSN: 0960-7420