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Literature DB >> 167670

Changes in phosphatase activity associated with cell wall defects in Chlamydomonas reinhardi.

Abstract

Experiments were performed to isolate mutants lacking alkaline phosphatase in Chlamydomonas reinhardi. Mutants with null enzyme activity were obtained. A cytological study of these mutants however revealed cell wall defects, suggesting that the loss of phosphatase activity in these strains is not due to the inactivation of the corresponding phosphatase structural gene but rather to the leakage of this enzyme as a consequence of the cell wall abnormality. Incidentally, this finding provides the basis of a convenient method for selecting easily cell wall mutants of Chlamydomonas.

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Year: 1975 PMID： 167670 DOI： 10.1007/bf00436357

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

6 in total

1. Leakage of periplasmic enzymes by mutants of Escherichia coli and Salmonella typhimurium: isolation of "periplasmic leaky" mutants.

Authors: J Lopes; S Gottfried; L Rothfield
Journal: J Bacteriol Date: 1972-02 Impact factor: 3.490

2. Acid phosphatase mutants in Chlamydomonas: isolation and characterization by biochemical, electrophoretic and genetic analysis.

Authors: R Loppes; R F Matagne
Journal: Genetics Date: 1973-12 Impact factor: 4.562

6. Chlamydomonas Genome Resource for Laboratory Strains Reveals a Mosaic of Sequence Variation, Identifies True Strain Histories, and Enables Strain-Specific Studies.

Authors: Sean D Gallaher; Sorel T Fitz-Gibbon; Anne G Glaesener; Matteo Pellegrini; Sabeeha S Merchant
Journal: Plant Cell Date: 2015-08-25 Impact factor: 11.277

6 in total

Changes in phosphatase activity associated with cell wall defects in Chlamydomonas reinhardi.

1. Leakage of periplasmic enzymes by mutants of Escherichia coli and Salmonella typhimurium: isolation of "periplasmic leaky" mutants.

2. Acid phosphatase mutants in Chlamydomonas: isolation and characterization by biochemical, electrophoretic and genetic analysis.

3. Alkaline phosphatase localization and spheroplast formation of Pseudomonas aeruginosa.

4. Isolation and study of mutants lacking a derepressible phosphatase in Chlamydomonas reinhardi.

5. Interactions of alkaline phosphatase and the cell wall of Pseudomonas aeruginosa.

6. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.

1. Phosphatase of Chlamydomonas reinhardi: biochemical and cytochemical approach with specific mutants.

2. Release of enzymes by normal and wall-free cells of Chlamydomonas.

3. Acid phosphatase isoenzymes of Chlamydomonas reinhardii.

4. Extracellular phosphatases of Chlamydomonas reinhardi and their regulation.

5. 14-3-3 proteins are constituents of the insoluble glycoprotein framework of the chlamydomonas cell wall.

6. Chlamydomonas Genome Resource for Laboratory Strains Reveals a Mosaic of Sequence Variation, Identifies True Strain Histories, and Enables Strain-Specific Studies.