Literature DB >> 16765841

Efficient transformation of Legionella pneumophila by high-voltage electroporation.

Ding-Qiang Chen1, Shao-Song Huang, Yong-Jun Lu.   

Abstract

A simple and reproducible method has been developed to transform Legionella pneumophila by electroporation. Effects of different conditions, including electric field strength, pulse length, DNA quality and cell density, were evaluated. Using our method, an efficiency of up to 6 x 10(7) transformants/microg DNA was obtained. This optimized transformation procedure should efficiently facilitate gene manipulations in L. pneumophila, such as plasmid transfer, transposon mutagenesis, library transformation for complementation cloning, etc.

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Year:  2005        PMID： 16765841     DOI： 10.1016/j.micres.2005.09.001

Source DB:  PubMed          Journal:  Microbiol Res        ISSN： 0944-5013            Impact factor:   5.415


  18 in total

1.  Analysis of the transcriptome of Legionella pneumophila hfq mutant reveals a new mobile genetic element.

Authors:  Hana Trigui; Paulina Dudyk; Janet Sum; Howard A Shuman; Sebastien P Faucher
Journal:  Microbiology       Date:  2013-05-31       Impact factor: 2.777

2.  Efficient generation of unmarked deletions in Legionella pneumophila.

Authors:  Andrew Bryan; Kaoru Harada; Michele S Swanson
Journal:  Appl Environ Microbiol       Date:  2011-02-04       Impact factor: 4.792

3.  A regulatory feedback loop between RpoS and SpoT supports the survival of Legionella pneumophila in water.

Authors:  Hana Trigui; Paulina Dudyk; Jinrok Oh; Jong-In Hong; Sebastien P Faucher
Journal:  Appl Environ Microbiol       Date:  2014-11-21       Impact factor: 4.792

4.  The Legionella effector RavD binds phosphatidylinositol-3-phosphate and helps suppress endolysosomal maturation of the Legionella-containing vacuole.

Authors:  Colleen M Pike; Rebecca Boyer-Andersen; Lisa N Kinch; Jeffrey L Caplan; M Ramona Neunuebel
Journal:  J Biol Chem       Date:  2019-02-07       Impact factor: 5.157

5.  Glucose metabolism in Legionella pneumophila: dependence on the Entner-Doudoroff pathway and connection with intracellular bacterial growth.

Authors:  Eiji Harada; Ken-Ichiro Iida; Susumu Shiota; Hiroaki Nakayama; Shin-Ichi Yoshida
Journal:  J Bacteriol       Date:  2010-04-02       Impact factor: 3.490

6.  Enzymatic properties of an ecto-nucleoside triphosphate diphosphohydrolase from Legionella pneumophila: substrate specificity and requirement for virulence.

Authors:  Fiona M Sansom; Patrice Riedmaier; Hayley J Newton; Michelle A Dunstone; Christa E Müller; Holger Stephan; Emma Byres; Travis Beddoe; Jamie Rossjohn; Peter J Cowan; Anthony J F d'Apice; Simon C Robson; Elizabeth L Hartland
Journal:  J Biol Chem       Date:  2008-03-12       Impact factor: 5.157

7.  Paradoxical Pro-inflammatory Responses by Human Macrophages to an Amoebae Host-Adapted Legionella Effector.

Authors:  Christopher Price; Snake Jones; Mirna Mihelcic; Marina Santic; Yousef Abu Kwaik
Journal:  Cell Host Microbe       Date:  2020-03-27       Impact factor: 21.023

8.  LtpD is a novel Legionella pneumophila effector that binds phosphatidylinositol 3-phosphate and inositol monophosphatase IMPA1.

Authors:  Clare R Harding; Corinna Mattheis; Aurélie Mousnier; Clare V Oates; Elizabeth L Hartland; Gad Frankel; Gunnar N Schroeder
Journal:  Infect Immun       Date:  2013-09-03       Impact factor: 3.441

9.  Legionella pneumophila Effector LpdA Is a Palmitoylated Phospholipase D Virulence Factor.

Authors:  Gunnar N Schroeder; Philipp Aurass; Clare V Oates; Edward W Tate; Elizabeth L Hartland; Antje Flieger; Gad Frankel
Journal:  Infect Immun       Date:  2015-07-27       Impact factor: 3.441

10.  Phylogenetic reconstruction of the Legionella pneumophila Philadelphia-1 laboratory strains through comparative genomics.

Authors:  Chitong Rao; Hadas Benhabib; Alexander W Ensminger
Journal:  PLoS One       Date:  2013-05-22       Impact factor: 3.240
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