Literature DB >> 16763847

Development of resistant transgenic soybeans with inverted repeat-coat protein genes of soybean dwarf virus.

Makoto Tougou1, Noriyuki Furutani, Noriko Yamagishi, Yoshiaki Shizukawa, Yoshihito Takahata, Soh Hidaka.   

Abstract

In an attempt to generate soybean plants resistant to soybean dwarf virus (SbDV), we transformed a construct containing inverted repeat-SbDV coat protein (CP) genes spaced by beta-glucuronidase (GUS) sequences into soybean somatic embryos via microprojectile bombardment. Three T(0) plants with an introduced CP gene were obtained, and one generated T(1) seeds. The presence of the transgene in T(1) plants was confirmed by PCR and Southern blot hybridization analysis, but expression of CP was not detected by northern blot hybridization analysis. Two months after inoculation of SbDV by aphid, T(2) plants contained little SbDV-specific RNA and remained symptomless. These plants contained SbDV-CP-specific siRNA. These results suggest that the T(2) plants achieved resistance to SbDV by an RNA-silencing-mediated process.

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Year:  2006        PMID: 16763847     DOI: 10.1007/s00299-006-0186-6

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN: 0721-7714            Impact factor:   4.570


  22 in total

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Authors:  H Terauchi; S Kanematsu; K Honda; Y Mikoshiba; K Ishiguro; S Hidaka
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10.  Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene.

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  15 in total

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Review 3.  Genetic improvement for phosphorus efficiency in soybean: a radical approach.

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6.  The application of the mutated acetolactate synthase gene from rice as the selectable marker gene in the production of transgenic soybeans.

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7.  Transgenic peppers that are highly tolerant to a new CMV pathotype.

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8.  Soybean dwarf virus-resistant transgenic soybeans with the sense coat protein gene.

Authors:  Makoto Tougou; Noriko Yamagishi; Noriyuki Furutani; Yoshiaki Shizukawa; Yoshihito Takahata; Soh Hidaka
Journal:  Plant Cell Rep       Date:  2007-07-14       Impact factor: 4.570

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Journal:  Plant Cell Rep       Date:  2017-07-29       Impact factor: 4.570

10.  RNA silencing as a tool to uncover gene function and engineer novel traits in soybean.

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