OBJECTIVE: To investigate whether the keloid susceptibility loci occur on chromosome 7p11 in a Chinese pedigree. METHODS: Peripheral venous blood samples from 32 members significant for hereditary investigation from a large Chinese keloid pedigree (with 5 affected generations) were collected for extracting genomic DNA. Four microsatellites on chromosome 7p11 with known maximal two-point LOD scores were selected as the genetic markers according to the method described in a recent report of the similar study. These markers were subsequently amplified by PCR, and all the PCR products were genotyped and linkage analyses were conducted. RESULTS: With the theta value of 0-0.1, the two-point LOD scores for these markers were all less than -2, which excluded the linkages of these markers to chromosome 7p11. CONCLUSION: This study provides the first genetic evidence that keloid susceptibility loci in Chinese pedigree do not locate on chromosome 7p11, suggesting the heterogeneity of familial keloid susceptibility loci.
OBJECTIVE: To investigate whether the keloid susceptibility loci occur on chromosome 7p11 in a Chinese pedigree. METHODS: Peripheral venous blood samples from 32 members significant for hereditary investigation from a large Chinese keloid pedigree (with 5 affected generations) were collected for extracting genomic DNA. Four microsatellites on chromosome 7p11 with known maximal two-point LOD scores were selected as the genetic markers according to the method described in a recent report of the similar study. These markers were subsequently amplified by PCR, and all the PCR products were genotyped and linkage analyses were conducted. RESULTS: With the theta value of 0-0.1, the two-point LOD scores for these markers were all less than -2, which excluded the linkages of these markers to chromosome 7p11. CONCLUSION: This study provides the first genetic evidence that keloid susceptibility loci in Chinese pedigree do not locate on chromosome 7p11, suggesting the heterogeneity of familial keloid susceptibility loci.