Literature DB >> 16760329

Expression of bovine viral diarrhea virus glycoprotein E2 as a soluble secreted form in a Mammalian cell line.

Gaetano Donofrio1, Ezio Bottarelli, Cavirani Sandro, Cesidio Filippo Flammini.   

Abstract

Bovine viral diarrhea virus (BVDV) membrane-anchored type I glycoprotein E2 is an approximately 53-kDa immunodominant glycoprotein inducing the production of neutralizing antibodies in the animal host after natural infection or following immunization with live or killed vaccines. The E2 coding region lacking the transmembrane domain was constructed in a soluble secreted form (secE2) and expressed in the medium of a transiently transfected human cell line. The crude conditioned medium containing secE2 can be potentially employed to develop an enzyme-linked immunosorbent assay antigen for the diagnosis of BVDV infection or for vaccine purposes.

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Year:  2006        PMID： 16760329      PMCID： PMC1489545          DOI： 10.1128/CVI.00071-06

Source DB:  PubMed          Journal:  Clin Vaccine Immunol        ISSN： 1556-679X


  10 in total

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3.  Molecular characterization of border disease virus, a pestivirus from sheep.

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Journal:  Virology       Date:  1994-02       Impact factor: 3.616

4.  Immunogens of bovine viral diarrhea virus.

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Journal:  Vet Microbiol       Date:  1993-11       Impact factor: 3.293

5.  Glycoprotein E2 of bovine viral diarrhea virus expressed in insect cells provides calves limited protection from systemic infection and disease.

Authors:  S R Bolin; J F Ridpath
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

6.  Processing of the envelope glycoproteins of pestiviruses.

Authors:  T Rümenapf; G Unger; J H Strauss; H J Thiel
Journal:  J Virol       Date:  1993-06       Impact factor: 5.103

7.  Hog cholera virus: molecular composition of virions from a pestivirus.

Authors:  H J Thiel; R Stark; E Weiland; T Rümenapf; G Meyers
Journal:  J Virol       Date:  1991-09       Impact factor: 5.103

8.  Molecular cloning and nucleotide sequence of the genome of hog cholera virus.

Authors:  G Meyers; T Rümenapf; H J Thiel
Journal:  Virology       Date:  1989-08       Impact factor: 3.616

9.  Further characterization of border disease virus isolates: evidence for the presence of more than three species within the genus pestivirus.

Authors:  P Becher; M König; D J Paton; H J Thiel
Journal:  Virology       Date:  1995-05-10       Impact factor: 3.616

Review 10.  Molecular biology of bovine viral diarrhea virus and its interactions with the host.

Authors:  R O Donis
Journal:  Vet Clin North Am Food Anim Pract       Date:  1995-11       Impact factor: 3.357
  10 in total
  8 in total

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4.  Pestivirus infection in cattle dairy farms: E2 glycoprotein ELISA reveals the presence of bovine viral diarrhea virus type 2 in northwestern Italy.

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5.  Assessment and optimization of Theileria parva sporozoite full-length p67 antigen expression in mammalian cells.

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6.  BVDV Npro protein mediates the BVDV induced immunosuppression through interaction with cellular S100A9 protein.

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7.  BoHV-4 immediate early 1 gene is a dispensable gene and its product is not a bone marrow stromal cell antigen 2 counteracting factor.

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8.  Development and validation of an indirect ELISA as a confirmatory test for surveillance of infectious bovine rhinotracheitis in vaccinated herds.

Authors:  Luigi Bertolotti; Elvira Muratore; Chiara Nogarol; Claudio Caruso; Laura Lucchese; Margherita Profiti; Laura Anfossi; Loretta Masoero; Stefano Nardelli; Sergio Rosati
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  8 in total

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