OBJECTIVE: To test the hypothesis that glycodelin is localized on pinopodes and correlates with temporal immunostaining of leukemia inhibitory factor (LIF), LIF receptor (LIFR), and progesterone receptor B (PRB). DESIGN: Prospective clinical study. SETTING: Hospital-based reproductive health unit and research laboratories. PATIENT(S): Twenty-five healthy fertile women with normal menstrual cycles. INTERVENTION(S): Endometrial biopsy specimens were obtained from healthy fertile women in the luteal phase of the menstrual cycle. MAIN OUTCOME MEASURE(S): Immunohistochemical staining of glycodelin, ultrastructural immunostaining of glycodelin, and double staining of glycodelin and PRB. RESULT(S): Glycodelin is present in the glands when pinopodes appear. Glycodelin is localized on pinopodes but is also secreted from luminal epithelial cells regardless of pinopode formation. There was a negative correlation between glycodelin secretion from the glands and PRB staining. A weak correlation between the presence of LIFR (but not LIF) and glycodelin was found. CONCLUSION(S): Pinopode appearance, intense staining of LIFR in pinopodes and glycodelin staining in the glands are synchronized events. Down-regulation of PRB in the endometrium is concomitant with the presence of glycodelin in the endometrium, suggesting interaction.
OBJECTIVE: To test the hypothesis that glycodelin is localized on pinopodes and correlates with temporal immunostaining of leukemia inhibitory factor (LIF), LIF receptor (LIFR), and progesterone receptor B (PRB). DESIGN: Prospective clinical study. SETTING: Hospital-based reproductive health unit and research laboratories. PATIENT(S): Twenty-five healthy fertile women with normal menstrual cycles. INTERVENTION(S): Endometrial biopsy specimens were obtained from healthy fertile women in the luteal phase of the menstrual cycle. MAIN OUTCOME MEASURE(S): Immunohistochemical staining of glycodelin, ultrastructural immunostaining of glycodelin, and double staining of glycodelin and PRB. RESULT(S): Glycodelin is present in the glands when pinopodes appear. Glycodelin is localized on pinopodes but is also secreted from luminal epithelial cells regardless of pinopode formation. There was a negative correlation between glycodelin secretion from the glands and PRB staining. A weak correlation between the presence of LIFR (but not LIF) and glycodelin was found. CONCLUSION(S): Pinopode appearance, intense staining of LIFR in pinopodes and glycodelin staining in the glands are synchronized events. Down-regulation of PRB in the endometrium is concomitant with the presence of glycodelin in the endometrium, suggesting interaction.
Authors: Richard E Leach; Philip Jessmon; Christos Coutifaris; Michael Kruger; Evan R Myers; Rouba Ali-Fehmi; Sandra A Carson; Richard S Legro; William D Schlaff; Bruce R Carr; Michael P Steinkampf; Susan Silva; Phyllis C Leppert; Linda Giudice; Michael P Diamond; D Randall Armant Journal: Hum Reprod Date: 2012-01-02 Impact factor: 6.918
Authors: Jie Yu; Sarah L Berga; Erika B Johnston-MacAnanny; Neil Sidell; Indrani C Bagchi; Milan K Bagchi; Robert N Taylor Journal: Endocrinology Date: 2016-04-01 Impact factor: 4.736