Literature DB >> 16758771

A high-performance liquid chromatography method for hypoxanthine determination in vitreous humour: application to estimation of post mortem interval.

J I Muñoz1, E Costas, M S Rodríguez-Calvo, J M Suárez-Peñaranda, M López-Rivadulla, L Concheiro.   

Abstract

We have developed a new technique to determine the concentration of hypoxanthine [Hx] in a reverse phase column using a modified high-performance liquid chromatography (HPLC) method that is faster and more reliable than those previously described. In this paper we present a formula for estimating the post mortem interval (PMI) based on this HPLC method by applying the inverse prediction method. The regression line obtained by changing the variables gives PMI = 0.183 [Hx] + 0.599 (PMI in hours, [Hx] in micromol/L, R2 = 0.531, P < 0.05).

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Year:  2006        PMID: 16758771     DOI: 10.1191/0960327106ht615oa

Source DB:  PubMed          Journal:  Hum Exp Toxicol        ISSN: 0960-3271            Impact factor:   2.903


  6 in total

1.  High variation in hypoxanthine determination after analytical treatment of vitreous humor samples.

Authors:  Ana Camba; Elena Lendoiro; Cristina Cordeiro; Isabel Martínez-Silva; María S Rodríguez-Calvo; Duarte N Vieira; José I Muñoz-Barús
Journal:  Forensic Sci Med Pathol       Date:  2014-08-15       Impact factor: 2.007

2.  IPMICALC: an Integrated Post-mortem Interval Calculator.

Authors:  Manuel Febrero Bande; Lucía Ordóñez Mayán; Cristina Cordeiro; Duarte Nuno Vieira; José Ignacio Muñoz Barús
Journal:  Forensic Sci Med Pathol       Date:  2022-10-06       Impact factor: 2.456

3.  Impact of euthanasia, dissection and postmortem delay on metabolic profile in mouse retina and RPE/choroid.

Authors:  Siyan Zhu; Michelle Yam; Yekai Wang; Jonathan D Linton; Allison Grenell; James B Hurley; Jianhai Du
Journal:  Exp Eye Res       Date:  2018-06-01       Impact factor: 3.467

4.  Postmortem Ocular Findings in the Optical Coherence Tomography Era: A Proof of Concept Study Based on Six Forensic Cases.

Authors:  Matteo Nioi; Pietro Emanuele Napoli; Roberto Demontis; Emanuela Locci; Maurizio Fossarello; Ernesto d'Aloja
Journal:  Diagnostics (Basel)       Date:  2021-02-28

5.  Application of Innovative TGA/Chemometric Approach for Forensic Purposes: The Estimation of the Time since Death in Contaminated Specimens.

Authors:  Roberta Risoluti; Giuseppina Gullifa; Vittorio Fineschi; Paola Frati; Stefano Materazzi
Journal:  Diagnostics (Basel)       Date:  2021-01-14

6.  Vitreous humor analysis for the detection of xenobiotics in forensic toxicology: a review.

Authors:  Fabien Bévalot; Nathalie Cartiser; Charline Bottinelli; Laurent Fanton; Jérôme Guitton
Journal:  Forensic Toxicol       Date:  2015-10-28       Impact factor: 4.096

  6 in total

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