Literature DB >> 16757217

Molecular characterization of recombinant Hepatitis B surface antigen from Chinese hamster ovary and Hansenula polymorpha cells by high-performance size exclusion chromatography and multi-angle laser light scattering.

Weibin Zhou1, Jingxiu Bi, Jan-Christer Janson, Yan Li, Yongdong Huang, Yan Zhang, Zhiguo Su.   

Abstract

The molecular weight and size of recombinant Hepatitis B surface antigen (HBsAg) derived from Chinese hamster ovary (CHO) and the Hansenula polymorph have been characterized by high-performance size exclusion chromatography with multi-angle laser light scattering (HPSEC-MALLS). The average molecular weight of CHO-derived HBsAg particle (CHO-rHBsAg) (4921 kDa) was higher than that of H. polymorpha yeast strain (Hans-rHBsAg) (3010 kDa). The size of CHO-rHBsAg (22.1 nm) is nearly the same as that of native HBsAg compared to 18.1 nm for Hans-rHBsAg. The average monomer numbers were found to be 155 for CHO-rHBsAg and 86 for Hans-rHBsAg, respectively. The data obtained support the assumption that the higher immunogenicity of CHO-derived HBsAg is related to its more favorable macromolecular assembly structure.

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Year:  2006        PMID： 16757217     DOI： 10.1016/j.jchromb.2006.03.064

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


  6 in total

1.  Antibody response to revaccination among adult non-responders to primary Hepatitis B vaccination in China.

Authors:  Li Zhang; Jiaye Liu; Jingjing Lu; Bingyu Yan; Lizhi Song; Li Li; Fuqiang Cui; Guomin Zhang; Fuzhen Wang; Xiaofeng Liang; Aiqiang Xu
Journal:  Hum Vaccin Immunother       Date:  2015-08-07       Impact factor: 3.452

2.  Persistence of immune memory among adults with normal and high antibody response to primary hepatitis B vaccination: Results from a five-year follow-up study in China.

Authors:  Wenlong Wu; Jingjing Lv; Jiaye Liu; Bingyu Yan; Yi Feng; Aiqiang Xu; Li Zhang
Journal:  Hum Vaccin Immunother       Date:  2018-07-23       Impact factor: 3.452

3.  Hepatitis B virus induces IL-23 production in antigen presenting cells and causes liver damage via the IL-23/IL-17 axis.

Authors:  Qinghong Wang; Jijun Zhou; Bei Zhang; Zhiqiang Tian; Jun Tang; Yanhua Zheng; Zemin Huang; Yi Tian; Zhengcai Jia; Yan Tang; Jennifer C van Velkinburgh; Qing Mao; Xiuwu Bian; Yifang Ping; Bing Ni; Yuzhang Wu
Journal:  PLoS Pathog       Date:  2013-06-27       Impact factor: 6.823

Review 4.  Virus-like particles as a highly efficient vaccine platform: diversity of targets and production systems and advances in clinical development.

Authors:  Natasha Kushnir; Stephen J Streatfield; Vidadi Yusibov
Journal:  Vaccine       Date:  2012-11-06       Impact factor: 3.641

Review 5.  Production of virus-like particles for vaccines.

Authors:  J Fuenmayor; F Gòdia; L Cervera
Journal:  N Biotechnol       Date:  2017-08-01       Impact factor: 5.079

6.  An account on the innovative path of cooperation with Prof. Zhiguo Su in bioseparation.

Authors:  Jan-Christer Janson
Journal:  Eng Life Sci       Date:  2021-06-07       Impact factor: 2.678
  6 in total

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