Literature DB >> 16757174

Cloning, in vitro expression and bioactivity of goose interferon-alpha.

Hong-Tao Li1, Bo Ma, Jing-Wei Mi, Hong-Yan Jin, Li-Na Xu, Jun-Wei Wang.   

Abstract

The cDNA of goose interferon-alpha (goIFN-alpha) was amplified from PHA stimulated PBMCs of goose by RT-PCR. The cDNA encodes a 30-amino acid signal peptide and a 161-amino acid mature protein, respectively. Recombinant mature goIFN-alpha (rgoIFN-alpha) expressed by prokaryotic and eukaryotic system possessed antiviral activity that was neutralized by rabbit anti-rgoIFN-alpha antibody in vitro. On the other hand, rgoIFN-alpha lacks intrinsic macrophage activating factor (MAF) activity, peripheral blood leukocyte-derived macrophages (PBLMs) could not produce nitric oxide (NO) by stimulate with rgoIFN-alpha as compared to stimulate with recombinant mature goIFN-gamma (rgoIFN-gamma) that was a powerful NO stimulant in vitro.

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Year:  2006        PMID： 16757174     DOI： 10.1016/j.cyto.2006.04.013

Source DB:  PubMed          Journal:  Cytokine        ISSN： 1043-4666            Impact factor:   3.861


  4 in total

Review 1.  Interferons and their receptors in birds: a comparison of gene structure, phylogenetic analysis, and cross modulation.

Authors:  Hao Zhou; Shun Chen; Mingshu Wang; Anchun Cheng
Journal:  Int J Mol Sci       Date:  2014-11-14       Impact factor: 5.923

2.  Cross-Species Antiviral Activity of Goose Interferons against Duck Plague Virus Is Related to Its Positive Self-Feedback Regulation and Subsequent Interferon Stimulated Genes Induction.

Authors:  Hao Zhou; Shun Chen; Qin Zhou; Yunan Wei; Mingshu Wang; Renyong Jia; Dekang Zhu; Mafeng Liu; Fei Liu; Qiao Yang; Ying Wu; Kunfeng Sun; Xiaoyue Chen; Anchun Cheng
Journal:  Viruses       Date:  2016-07-18       Impact factor: 5.048

Review 3.  Avian Interferons and Their Antiviral Effectors.

Authors:  Diwakar Santhakumar; Dennis Rubbenstroth; Luis Martinez-Sobrido; Muhammad Munir
Journal:  Front Immunol       Date:  2017-01-31       Impact factor: 7.561

4.  The Arabian camel, Camelus dromedarius interferon epsilon: Functional expression, in vitro refolding, purification and cytotoxicity on breast cancer cell lines.

Authors:  Manal Abdel-Fattah; Hesham Saeed; Lamiaa El-Shennawy; Manal Shalaby; Amira Embaby; Farid Ataya; Hoda Mahmoud; Ahmed Hussein
Journal:  PLoS One       Date:  2019-09-06       Impact factor: 3.240
  4 in total

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