| Literature DB >> 16755037 |
Takashi Nakamura1, Rumiko Moriguchi, Kentaro Kogure, Arisa Minoura, Tomoya Masuda, Hidetaka Akita, Kazunori Kato, Hirofumi Hamada, Masaharu Ueno, Shiroh Futaki, Hideyoshi Harashima.
Abstract
In this study, we developed novel double-membranous non-viral gene delivery system modified with SV-40 T antigen-derived nuclear localization signal (NLS-DMEND) for delivery of luciferase plasmid DNA to nucleus of non-dividing mouse bone marrow-derived dendritic cells (BMDC). Intracellular trafficking and gene expression of NLS-DMEND in the BMDC were evaluated. Condensed DNA was observed in the nucleus by confocal laser scanning microscopy, and the NLS-DMEND induced significant luciferase activity in the BMDC. It was suggested that the condensed DNA particle transferred into nucleus via energy dependent manner, since the nuclear transfer was inhibited by metabolic inhibitors. In conclusion, condensed plasmid DNA was delivered into the nucleus of non-dividing BMDC by NLS-DMEND.Entities:
Mesh：
Substances：
Year: 2006 PMID： 16755037 DOI： 10.1248/bpb.29.1290
Source DB: PubMed Journal: Biol Pharm Bull ISSN： 0918-6158 Impact factor: 2.233