Literature DB >> 16754995

Purification and crystallization of the catalytic PRONE domain of RopGEF8 and its complex with Rop4 from Arabidopsis thaliana.

Christoph Thomas1, Michael Weyand, Alfred Wittinghofer, Antje Berken.   

Abstract

The PRONE domain of the guanine nucleotide exchange factor RopGEF8 (PRONE8) was purified and crystallized free and in complex with the Rho-family protein Rop4 using the hanging-drop vapour-diffusion method. PRONE8 crystals were obtained using NaCl as precipitating agent and belong to the hexagonal space group P6(5)22. Native and anomalous data sets were collected using synchrotron radiation at 100 K to 2.2 and 2.8 A resolution, respectively. Crystals of the Rop4-PRONE8 complex belonging to space group P6(3) were obtained using Tacsimate and PEG 3350 as precipitating agents and diffracted to 3.1 A resolution.

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Year:  2006        PMID： 16754995      PMCID： PMC2243088          DOI： 10.1107/S1744309106018689

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  14 in total

1.  Arabidopsis RopGAPs are a novel family of rho GTPase-activating proteins that require the Cdc42/Rac-interactive binding motif for rop-specific GTPase stimulation.

Authors:  G Wu; H Li; Z Yang
Journal:  Plant Physiol       Date:  2000-12       Impact factor: 8.340

Review 2.  The Rop GTPase: an emerging signaling switch in plants.

Authors:  Z L Zheng; Z Yang
Journal:  Plant Mol Biol       Date:  2000-09       Impact factor: 4.076

Review 3.  Dbl family guanine nucleotide exchange factors.

Authors:  Y Zheng
Journal:  Trends Biochem Sci       Date:  2001-12       Impact factor: 13.807

Review 4.  ROP/RAC GTPase: an old new master regulator for plant signaling.

Authors:  Ying Gu; Zonghua Wang; Zhenbiao Yang
Journal:  Curr Opin Plant Biol       Date:  2004-10       Impact factor: 7.834

5.  Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth.

Authors:  Pulla Kaothien; Sung Han Ok; Bin Shuai; Diego Wengier; Robyn Cotter; Dior Kelley; Stephanie Kiriakopolos; Jorge Muschietti; Sheila McCormick
Journal:  Plant J       Date:  2005-05       Impact factor: 6.417

6.  A new family of RhoGEFs activates the Rop molecular switch in plants.

Authors:  Antje Berken; Christoph Thomas; Alfred Wittinghofer
Journal:  Nature       Date:  2005-06-26       Impact factor: 49.962

7.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

Review 8.  The guanine nucleotide-binding switch in three dimensions.

Authors:  I R Vetter; A Wittinghofer
Journal:  Science       Date:  2001-11-09       Impact factor: 47.728

Review 9.  Structural elements, mechanism, and evolutionary convergence of Rho protein-guanine nucleotide exchange factor complexes.

Authors:  Jon W Erickson; Richard A Cerione
Journal:  Biochemistry       Date:  2004-02-03       Impact factor: 3.162

10.  Members of a novel class of Arabidopsis Rho guanine nucleotide exchange factors control Rho GTPase-dependent polar growth.

Authors:  Ying Gu; Shundai Li; Elizabeth M Lord; Zhenbiao Yang
Journal:  Plant Cell       Date:  2006-01-13       Impact factor: 11.277
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  1 in total

1.  Purification, crystallization and preliminary X-ray diffraction analysis of the plant Rho protein ROP5.

Authors:  Christoph Thomas; Antje Berken
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-11-30
  1 in total

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