| Literature DB >> 16750859 |
Rebecca L Nolan1, Jan K Teller.
Abstract
Representative extraction of both RNA and protein from a single biological sample is required for reliable assessment of coordinated changes in gene and protein expression. Such a simultaneous extraction can be performed by using Trizol Reagent. Here, we demonstrate that, as an alternative to SDS, 2% diethylamine is an effective solvent, which can be conveniently used in extraction of Trizol-isolated proteins from various tissues. Diethylamine provides efficient extraction of proteins and compatibility with a variety of common downstream analytical applications.Entities:
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Year: 2006 PMID： 16750859 DOI： 10.1016/j.jbbm.2006.04.002
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X