Literature DB >> 16750160

A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays.

Mathias Hampf1, Manfred Gossen.   

Abstract

We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay system was formulated under the aspect of full compatibility with standard methods for protein assays. This feature greatly expands the range of applications for assay systems quantifying the expression of multiple luciferase reporters.

Mesh：

Substances：

Year:  2006        PMID： 16750160     DOI： 10.1016/j.ab.2006.04.046

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  45 in total

1.  Conservation of a unique mechanism of immune evasion across the Lyssavirus genus.

Authors:  L Wiltzer; F Larrous; S Oksayan; N Ito; G A Marsh; L F Wang; D Blondel; H Bourhy; D A Jans; G W Moseley
Journal:  J Virol       Date:  2012-06-27       Impact factor: 5.103

2.  Homozygous missense and nonsense mutations in BMPR1B cause acromesomelic chondrodysplasia-type Grebe.

Authors:  Luitgard M Graul-Neumann; Alexandra Deichsel; Ulrike Wille; Naseebullah Kakar; Randi Koll; Christian Bassir; Jamil Ahmad; Valerie Cormier-Daire; Stefan Mundlos; Christian Kubisch; Guntram Borck; Eva Klopocki; Thomas D Mueller; Sandra C Doelken; Petra Seemann
Journal:  Eur J Hum Genet       Date:  2013-10-16       Impact factor: 4.246

3.  ACVR1 p.Q207E causes classic fibrodysplasia ossificans progressiva and is functionally distinct from the engineered constitutively active ACVR1 p.Q207D variant.

Authors:  Julia Haupt; Alexandra Deichsel; Katja Stange; Cindy Ast; Renata Bocciardi; Roberto Ravazzolo; Maja Di Rocco; Paola Ferrari; Antonio Landi; Frederick S Kaplan; Eileen M Shore; Carsten Reissner; Petra Seemann
Journal:  Hum Mol Genet       Date:  2014-05-22       Impact factor: 6.150

4.  Quantitative in vivo dual-color bioluminescence imaging in the mouse brain.

Authors:  Markus Aswendt; Stefanie Vogel; Cordula Schäfer; Amit Jathoul; Martin Pule; Mathias Hoehn
Journal:  Neurophotonics       Date:  2019-05-07       Impact factor: 3.593

5.  Design, Optimization, and Study of Small Molecules That Target Tau Pre-mRNA and Affect Splicing.

Authors:  Jonathan L Chen; Peiyuan Zhang; Masahito Abe; Haruo Aikawa; Liying Zhang; Alexander J Frank; Timothy Zembryski; Christopher Hubbs; HaJeung Park; Jane Withka; Claire Steppan; Lucy Rogers; Shawn Cabral; Martin Pettersson; Travis T Wager; Matthew A Fountain; Gavin Rumbaugh; Jessica L Childs-Disney; Matthew D Disney
Journal:  J Am Chem Soc       Date:  2020-05-04       Impact factor: 15.419

6.  High-throughput functional screening using a homemade dual-glow luciferase assay.

Authors:  Jessica M Baker; Frederick M Boyce
Journal:  J Vis Exp       Date:  2014-06-01       Impact factor: 1.355

7.  Cross-talk between the androgen receptor and the liver X receptor: implications for cholesterol homeostasis.

Authors:  James Robert Krycer; Andrew John Brown
Journal:  J Biol Chem       Date:  2011-04-13       Impact factor: 5.157

8.  Precise small-molecule recognition of a toxic CUG RNA repeat expansion.

Authors:  Suzanne G Rzuczek; Lesley A Colgan; Yoshio Nakai; Michael D Cameron; Denis Furling; Ryohei Yasuda; Matthew D Disney
Journal:  Nat Chem Biol       Date:  2016-12-12       Impact factor: 15.040

9.  Improved Tet-responsive promoters with minimized background expression.

Authors:  Rainer Loew; Niels Heinz; Mathias Hampf; Hermann Bujard; Manfred Gossen
Journal:  BMC Biotechnol       Date:  2010-11-24       Impact factor: 2.563

10.  Rearrangement of the Extracellular Domain/Extracellular Loop 1 Interface Is Critical for Thyrotropin Receptor Activation.

Authors:  Joerg Schaarschmidt; Marcus B M Nagel; Sandra Huth; Holger Jaeschke; Rocco Moretti; Vera Hintze; Martin von Bergen; Stefan Kalkhof; Jens Meiler; Ralf Paschke
Journal:  J Biol Chem       Date:  2016-04-26       Impact factor: 5.157
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.