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Literature DB >> 16749157

Lactate dehydrogenase isoenzymes of human semen.

Abstract

1. A lactate dehydrogenase isoenzyme present in human spermatozoa and semen was isolated and characterized biochemically in term of its pH for optimum activity and by means of K(m) values for lactate, NAD(+) and NAD analogues. The results were compared with those obtained with the human heart-type and the liver-type lactate dehydrogenase isoenzymes. 2. The enzyme was characterized by its resistance to digestion with different proteolytic enzymes. The time for 50% digestion in terms of residual dehydrogenase activity was compared with times obtained for the H(4)- and M(4)-types.

Entities: Chemical Species

Year: 1965 PMID： 16749157 PMCID： PMC1264668 DOI： 10.1042/bj0970513

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

16 in total

1. THE COMPARATIVE ENZYMOLOGY OF LACTIC DEHYDROGENASES. I. PROPERTIES OF THE CRYSTALLINE BEEF AND CHICKEN ENZYMES.

Authors: A PESCE; R H MCKAY; F STOLZENBACH; R D CAHN; N O KAPLAN
Journal: J Biol Chem Date: 1964-06 Impact factor: 5.157

2. LACTATE DEHYDROGENASE IN TESTIS: DISSOCIATION AND RECOMBINATION OF SUBUNITS.

Authors: W H ZINKHAM; A BLANCO; L KUPCHYK
Journal: Science Date: 1963-12-06 Impact factor: 47.728

3. Evolution and differentiation of dehvdrogenases.

Authors: N O KAPLAN; M M CIOTTI
Journal: Ann N Y Acad Sci Date: 1961-11-02 Impact factor: 5.691

4. Changes of LDH-isozymes, esterases, acid phosphatases and proteins in malignant and benign human brain tumors.

Authors: W Gerhardt; J Clausen; E Christensen; J Rushede
Journal: Acta Neurol Scand Date: 1963 Impact factor: 3.209

5. Starch gel electrophoresis of lactic dehydrogenase from rat kidney.

Authors: M M DEWEY; J L CONKLIN
Journal: Proc Soc Exp Biol Med Date: 1960-12

6. [Enzymic determination of L(+)-lactic acid].

Authors: H J HOHORST
Journal: Biochem Z Date: 1957

7. [Colorimetric determination of total proteins and globulin in spinal fluid; trial of Lowry's method].

Authors: P LOUIS; C M PLUM; M SCHOU
Journal: Nord Med Date: 1956-05-17

8. A microchemical determination of desoxyribonucleic acid.

Authors: G CERIOTTI
Journal: J Biol Chem Date: 1952-09 Impact factor: 5.157

9. MULTIPLE FORMS OF ENZYMES: TISSUE, ONTOGENETIC, AND SPECIES SPECIFIC PATTERNS.

Authors: C L Markert; F Møller
Journal: Proc Natl Acad Sci U S A Date: 1959-05 Impact factor: 11.205

10. Lactic dehydrogenases: subfractionation of isozymes.

Authors: P J FRITZ; K B JACOBSON
Journal: Science Date: 1963-04-05 Impact factor: 47.728

15 in total

1. Effect of fat-free diet on enzyme activities of rat testes.

Authors: J Clausen
Journal: Z Ernahrungswiss Date: 1969-07

2. Uptake and distribution in rat brain of organic and inorganic selenium.

Authors: J Clausen
Journal: Biol Trace Elem Res Date: 1991-01 Impact factor: 3.738

3. Glycolytic enzymes in mammalian spermatozoa. Activities and stabilities of hexokinase and phosphofructokinase in various fractions from sperm homogenates.

Authors: R A Harrison
Journal: Biochem J Date: 1971-10 Impact factor: 3.857

4. Factors affecting the lactate dehydrogenase isoenzyme pattern of cultured kidney-cortex cells.

Authors: F Güttler; J Clausen
Journal: Biochem J Date: 1969-10 Impact factor: 3.857

5. The foetal development of lactate dehydrogenase isoenzymes, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from human striated muscle.

Authors: J Clausen; R Hustrulid
Journal: Biochem J Date: 1969-01 Impact factor: 3.857

6. Comperative studies with Culex pipiens egg rafts. Immunogenetic, electrophoretic and enzymatic analysis of unfertilized, compatible and incompatible fertilized eggs.

Authors: W Schumann
Journal: Theor Appl Genet Date: 1974-01 Impact factor: 5.699