Literature DB >> 1674912

New assays for the tyrosine hydroxylase and dopa oxidase activities of tyrosinase.

A J Winder1, H Harris.   

Abstract

New assays for the tyrosine hydroxylase and dopa oxidase activities of tyrosinase (EC 1.14.18.1) have been developed. The tyrosine hydroxylase assay uses L-[carboxy-14C]tyrosine as the substrate, 14CO2 is released from the products of the hydroxylation and further metabolism of L-[carboxy-14C]tyrosine by incubation with ferricyanide, and measured radiometrically. D-Dopa is a preferable cofactor to L-dopa for the assay. Dopa oxidase activity is measured spectrophotometrically. Dopaquinone, produced on the oxidation of L-dopa, reacts with Besthorn's hydrazone (3-methyl-2-benzothiazolinone hydrazone) to form a pink pigment with an absorbance maximum at 505 nm. Details of the optimisation of conditions for the assays and their specificities for the two enzyme activities are described.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1674912     DOI： 10.1111/j.1432-1033.1991.tb16018.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  45 in total

1.  Tyrosinases from crustaceans form hexamers.

Authors:  Elmar Jaenicke; Heinz Decker
Journal:  Biochem J       Date:  2003-04-15       Impact factor: 3.857

2.  A new continuous spectrophotometric assay method for DOPA oxidase activity of tyrosinase.

Authors:  Yong-Doo Park; Jae-Rin Lee; Kyung-Hee Park; Hwa-Sun Hahn; Myong-Joon Hahn; Jun-Mo Yang
Journal:  J Protein Chem       Date:  2003-07

3.  Membrane insertion exacerbates the α-Synuclein-Cu(II) dopamine oxidase activity: Metallothionein-3 targets and silences all α-synuclein-Cu(II) complexes.

Authors:  Jenifer S Calvo; Neha V Mulpuri; Alex Dao; Nabeeha K Qazi; Gabriele Meloni
Journal:  Free Radic Biol Med       Date:  2020-07-23       Impact factor: 7.376

4.  Tyrosinase activity and hemocyanin in the hemolymph of the slipper lobster Scyllarides latus.

Authors:  Alessandra Olianas; Enrico Sanjust; Mariagiuseppina Pellegrini; Antonio Rescigno
Journal:  J Comp Physiol B       Date:  2005-07-12       Impact factor: 2.200

5.  Glyphosate inhibits melanization and increases susceptibility to infection in insects.

Authors:  Daniel F Q Smith; Emma Camacho; Raviraj Thakur; Alexander J Barron; Yuemei Dong; George Dimopoulos; Nichole A Broderick; Arturo Casadevall
Journal:  PLoS Biol       Date:  2021-05-12       Impact factor: 8.029

6.  Human tyrosinase produced in insect cells: a landmark for the screening of new drugs addressing its activity.

Authors:  Stefano Fogal; Marcello Carotti; Laura Giaretta; Federico Lanciai; Leonardo Nogara; Luigi Bubacco; Elisabetta Bergantino
Journal:  Mol Biotechnol       Date:  2015-01       Impact factor: 2.695

7.  Oxidative stress level and tyrosinase activity in vitiligo patients.

Authors:  M Eskandani; J Golchai; N Pirooznia; S Hasannia
Journal:  Indian J Dermatol       Date:  2010       Impact factor: 1.494

8.  Color reduction of melanin by lysosomal and peroxisomal enzymes isolated from mammalian cells.

Authors:  Dong Jun Park; Simranjeet Singh Sekhon; Jihee Yoon; Yang-Hoon Kim; Jiho Min
Journal:  Mol Cell Biochem       Date:  2016-01-06       Impact factor: 3.396

9.  Biosynthesis and cytoplasmic accumulation of a chlorinated catechol pigment during 3-chlorobenzoate aerobic co-metabolism in Pseudomonas fluorescens.

Authors:  F Fava; D Di Gioia; C Romagnoli; L Marchetti; D Mares
Journal:  Arch Microbiol       Date:  1993       Impact factor: 2.552

10.  PPAR gamma regulates MITF and beta-catenin expression and promotes a differentiated phenotype in mouse melanoma S91.

Authors:  Maja Grabacka; Wojciech Placha; Krystyna Urbanska; Piotr Laidler; Przemysław M Płonka; Krzysztof Reiss
Journal:  Pigment Cell Melanoma Res       Date:  2008-04-26       Impact factor: 4.693
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.