Literature DB >> 1674765

DNA amplification on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia.

A E Wakefield1, L Guiver, R F Miller, J M Hopkin.   

Abstract

DNA amplification and silver staining were used to identify Pneumocystis carinii in bronchoscopic lavage and induced sputum samples during 51 episodes of respiratory illness in 47 subjects with HIV infection. In 20 episodes, in which the clinical diagnosis was pneumocystis pneumonia (PCP), silver stain was positive in 14 lavage samples (70%) and 7 sputum samples (35%), whereas DNA amplification was positive in 19 lavage samples (95%) and 18 sputum samples (90%). DNA amplification was positive in only 1 of 31 patients without PCP (PCP developed in this patient within 10 weeks). DNA amplification on induced sputum offers a powerful technique for diagnosis of PCP.

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Year:  1991        PMID： 1674765     DOI： 10.1016/0140-6736(91)93062-e

Source DB:  PubMed          Journal:  Lancet        ISSN： 0140-6736            Impact factor:   79.321


  44 in total

Review 1.  Laboratory investigation of Pneumocystis carinii pneumonia.

Authors:  J M Chatterton; D O Yen
Journal:  Genitourin Med       Date:  1992-10

Review 2.  Respiratory diseases.

Authors:  D G James
Journal:  Postgrad Med J       Date:  1992-03       Impact factor: 2.401

Review 3.  AIDS and the lung: update 1992. 1. Pneumocystis carinii pneumonia.

Authors:  R F Miller; D M Mitchell
Journal:  Thorax       Date:  1992-04       Impact factor: 9.139

4.  Trimethoprim-sulfamethoxazole treatment does not reverse obstructive pulmonary changes in pneumocystis-colonized nonhuman primates with SHIV infection.

Authors:  Heather M Kling; Timothy W Shipley; Siobhan Guyach; Rebecca Tarantelli; Alison Morris; Karen A Norris
Journal:  J Acquir Immune Defic Syndr       Date:  2014-04-01       Impact factor: 3.731

5.  Comparison of PCR, (1-->3)-beta-D-glucan and galactomannan assays in sera of rats with experimental invasive aspergillosis.

Authors:  A Hashimoto; Y Yamakami; P Kamberi; E Yamagata; R Karashima; H Nagaoka; M Nasu
Journal:  J Clin Lab Anal       Date:  1998       Impact factor: 2.352

Review 6.  South African guideline for the management of community-acquired pneumonia in adults.

Authors:  Tom H Boyles; Adrian Brink; Greg L Calligaro; Cheryl Cohen; Keertan Dheda; Gary Maartens; Guy A Richards; Richard van Zyl Smit; Clifford Smith; Sean Wasserman; Andrew C Whitelaw; Charles Feldman
Journal:  J Thorac Dis       Date:  2017-06       Impact factor: 2.895

7.  Development and evaluation of a quantitative, touch-down, real-time PCR assay for diagnosing Pneumocystis carinii pneumonia.

Authors:  Hans Henrik Larsen; Henry Masur; Joseph A Kovacs; Vee J Gill; Victoria A Silcott; Palaniandy Kogulan; Janine Maenza; Margo Smith; Daniel R Lucey; Steven H Fischer
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

8.  Use of semiquantitative PCR to assess onset and treatment of Pneumocystis carinii infection in rat model.

Authors:  T J O'Leary; M M Tsai; C F Wright; M T Cushion
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

9.  Reduced carbon monoxide transfer factor (TLCO) in human immunodeficiency virus type I (HIV-I) infection as a predictor for faster progression to AIDS.

Authors:  R B Nieman; J Fleming; R J Coker; J R Harris; D M Mitchell
Journal:  Thorax       Date:  1993-05       Impact factor: 9.139

10.  Serum markers in interstitial pneumonia with and without Pneumocystis jirovecii colonization: a prospective study.

Authors:  Yasuo Shimizu; Noriaki Sunaga; Kunio Dobashi; Makoto Fueki; Naoto Fueki; Sohei Makino; Masatomo Mori
Journal:  BMC Infect Dis       Date:  2009-04-22       Impact factor: 3.090
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