Marcio C Bajgelman1, Bryan E Strauss. 1. Viral Vector Group, Laboratory of Genetics and Molecular Cardiology, InCor, University of São Paulo School of Medicine, Brazil.
Abstract
BACKGROUND: Some nuances of mutant and wild-type p53 activity have been uncovered utilizing temperature sensitive (TS) alleles. However, few human tumor derived cell lines possess a TS p53 mutant. METHODS: The cell lines DU145 (heterozygous p53, P223L, and V274F) and PC3 (p53-null, where exogenous P223L and V274F were introduced individually or in combination) were examined for TS p53 activity as revealed by reporter construct and target gene activation. RESULTS: TS p53 function was observed in DU145 and expression of the P223L allele in PC3 conferred a TS p53 profile. Activation of p21Waf1 demonstrated that P223L TS activity may have been influenced by cellular context. CONCLUSIONS: The DU145 cell line harbors a TS mutant of p53 and, in addition to being a widely used model of human prostate carcinoma, may also reveal new insights into p53 function due to the unique transcriptional properties of its TS phenotype.
BACKGROUND: Some nuances of mutant and wild-type p53 activity have been uncovered utilizing temperature sensitive (TS) alleles. However, few humantumor derived cell lines possess a TS p53 mutant. METHODS: The cell lines DU145 (heterozygous p53, P223L, and V274F) and PC3 (p53-null, where exogenous P223L and V274F were introduced individually or in combination) were examined for TS p53 activity as revealed by reporter construct and target gene activation. RESULTS: TS p53 function was observed in DU145 and expression of the P223L allele in PC3 conferred a TS p53 profile. Activation of p21Waf1 demonstrated that P223L TS activity may have been influenced by cellular context. CONCLUSIONS: The DU145 cell line harbors a TS mutant of p53 and, in addition to being a widely used model of humanprostate carcinoma, may also reveal new insights into p53 function due to the unique transcriptional properties of its TS phenotype.
Authors: Bradley T Scroggins; Masayuki Matsuo; Ayla O White; Keita Saito; Jeeva P Munasinghe; Carole Sourbier; Kazutoshi Yamamoto; Vivian Diaz; Yoichi Takakusagi; Kazuhiro Ichikawa; James B Mitchell; Murali C Krishna; Deborah E Citrin Journal: Clin Cancer Res Date: 2018-03-29 Impact factor: 12.531
Authors: Christian A Merkel; Rafael B da Silva Soares; Anna Carolina V de Carvalho; Daniela B Zanatta; Marcio C Bajgelman; Paula Fratini; Eugenia Costanzi-Strauss; Bryan E Strauss Journal: BMC Cancer Date: 2010-06-22 Impact factor: 4.430
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