Comparison of gene expression profiles in mouse primary T cells under normal and prolonged activation.

In order to investigate the global transcriptional change of mouse primary T cells after prolonged activation, we took advantage of a Mouse Genome 430 2.0 Array to assess and compare the overall gene expression profiles of mouse T cells after activated with anti-CD3/CD28 for 18 or 48 h. The results demonstrated that most activation-related genes were preferentially up-regulated in mouse primary T cells after stimulated for 18 h; some apoptotic genes, however, were also found to be moderately up-regulated simultaneously. After the activation of T cells for 48 h, lots of apoptosis-related genes were dramatically up-regulated, followed by the augmentation of activation-induced cell death. In general, the number of differentially expressed genes in T cells after activation over 48 h declined almost in half as compared to that of 18 h. Both microarray and cytokine content analyses revealed that Th1 cytokines, rather than Th2 cytokines, were specifically up-regulated in activated mouse primary T cells. The present study also identified a number of genes that were dramatically up or down-regulated in T cells activated for 48 h for the first time, although the exact functions of these proteins are not known. Our studies provide detailed information on genes expression profiles of mouse primary T cells after normal (18 h) and prolonged activation (48 h); these data may accelerate the understanding of the T cell activation process and offer clues to the therapy of immune diseases.