| Literature DB >> 16740304 |
David Epel1, Bryan Cole, Amro Hamdoun, Rebecca Vega Thurber.
Abstract
We describe the use of the sea urchin as a model for studying efflux transporters and estimating energy cost for the cytotoxin protective system provided by these transporters. The unfertilized egg has low transport activity, which increases to a new steady state shortly after fertilization. Activity results from p-glycoprotein (p-gp) and MRP type transporters which protect the embryo from cytotoxic drugs that can disrupt cell division or induce apoptosis. The energy cost is estimated from a novel use of calcein-AM as a substrate; keeping 0.25 microM substrate levels out of the cell utilizes only 0.023% of steady state respiration.Entities:
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Year: 2006 PMID： 16740304 PMCID： PMC3160780 DOI： 10.1016/j.marenvres.2006.04.062
Source DB: PubMed Journal: Mar Environ Res ISSN： 0141-1136 Impact factor: 3.130