| Literature DB >> 16739716 |
Xavier Altafaj1, Nathalie Joux, Michel Ronjat, Michel De Waard.
Abstract
The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.Entities:
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Year: 2006 PMID： 16739716 DOI： 10.1007/978-1-59745-000-3_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745