Literature DB >> 16735592

Heat-induced antigen retrieval: what are we retrieving?

Thomas Boenisch1.   

Abstract

Of all molecular aspects lost and recovered during formalin fixation and antigen retrieval, respectively, electrostatic charges have probably received the least attention. This review will focus on our work during the past 7 years. It strongly supports the tenet that electrostatic charges, i.e., net negative on antigens and net positive on antibodies, play an important part in immune reactions and therefore should be given greater attention when focusing on quality control in immunohistochemistry.

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Year:  2006        PMID： 16735592     DOI： 10.1369/jhc.6P6945.2006

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  7 in total

1.  Selection of buffer pH by the isoelectric point of the antigen for the efficient heat-induced epitope retrieval: re-appraisal for nuclear protein pathobiology.

Authors:  Hanako Kajiya; Susumu Takekoshi; Mao Takei; Noboru Egashira; Takashi Miyakoshi; Akihito Serizawa; Akira Teramoto; Robert Y Osamura
Journal:  Histochem Cell Biol       Date:  2009-09-19       Impact factor: 4.304

2.  Antigen Masking During Fixation and Embedding, Dissected.

Authors:  Carla Rossana Scalia; Giovanna Boi; Maddalena Maria Bolognesi; Lorella Riva; Marco Manzoni; Linde DeSmedt; Francesca Maria Bosisio; Susanna Ronchi; Biagio Eugenio Leone; Giorgio Cattoretti
Journal:  J Histochem Cytochem       Date:  2016-10-23       Impact factor: 2.479

3.  Specificity controls for immunocytochemistry: the antigen preadsorption test can lead to inaccurate assessment of antibody specificity.

Authors:  Silvia Holmseth; Yun Zhou; Virginie V Follin-Arbelet; Knut Petter Lehre; Dwight E Bergles; Niels Christian Danbolt
Journal:  J Histochem Cytochem       Date:  2012-01-03       Impact factor: 2.479

4.  Antigen retrieval causes protein unfolding: evidence for a linear epitope model of recovered immunoreactivity.

Authors:  Carol B Fowler; David L Evers; Timothy J O'Leary; Jeffrey T Mason
Journal:  J Histochem Cytochem       Date:  2011-04       Impact factor: 2.479

5.  Reduction of high background staining by heating unfixed mouse skeletal muscle tissue sections allows for detection of thermostable antigens with murine monoclonal antibodies.

Authors:  Rustam R Mundegar; Elke Franke; Ralf Schäfer; Margit Zweyer; Anton Wernig
Journal:  J Histochem Cytochem       Date:  2008-07-21       Impact factor: 2.479

6.  Mechanical Compression of Coverslipped Tissue Sections During Heat-induced Antigen Retrieval Prevents Section Detachment and Preserves Tissue Morphology.

Authors:  Andreas H Eckhard; Jennifer T O'Malley; Joseph B Nadol; Joe C Adams
Journal:  J Histochem Cytochem       Date:  2019-01-29       Impact factor: 2.479

7.  The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation.

Authors:  Guray Akturk; Michael Angelo; Elizabeth L Engle; Sacha Gnjatic; Shirley Greenbaum; Noah F Greenwald; Cyrus V Hedvat; Travis J Hollmann; Jonathan Juco; Edwin R Parra; Marlon C Rebelatto; David L Rimm; Jaime Rodriguez-Canales; Kurt A Schalper; Edward C Stack; Janis M Taube; Cláudia S Ferreira; Konstanty Korski; Ana Lako; Scott J Rodig; Emanuel Schenck; Keith E Steele; Michael J Surace; Michael T Tetzlaff; Katharina von Loga; Ignacio I Wistuba; Carlo B Bifulco
Journal:  J Immunother Cancer       Date:  2020-05       Impact factor: 13.751
  7 in total

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