Literature DB >> 16732729

Renaturation, activation, and practical use of recombinant duplex-specific nuclease from Kamchatka crab.

V E Anisimova1, D V Rebrikov, P A Zhulidov, D B Staroverov, S A Lukyanov, A S Shcheglov.   

Abstract

We overexpressed duplex-specific nuclease (DSN) from Kamchatka crab in Escherichia coli cells and developed procedures for purification, renaturation, and activation of this protein. We demonstrated identity of the properties of the native and recombinant DSN. We also successfully applied the recombinant DSN for full-length cDNA library normalization.

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Year:  2006        PMID： 16732729     DOI： 10.1134/s0006297906050075

Source DB:  PubMed          Journal:  Biochemistry (Mosc)        ISSN： 0006-2979            Impact factor:   2.487


  4 in total

Review 1.  Capturing the 'ome': the expanding molecular toolbox for RNA and DNA library construction.

Authors:  Morgane Boone; Andries De Koker; Nico Callewaert
Journal:  Nucleic Acids Res       Date:  2018-04-06       Impact factor: 16.971

2.  The Impact of cDNA Normalization on Long-Read Sequencing of a Complex Transcriptome.

Authors:  Nam V Hoang; Agnelo Furtado; Virginie Perlo; Frederik C Botha; Robert J Henry
Journal:  Front Genet       Date:  2019-07-23       Impact factor: 4.599

3.  A new set of ESTs and cDNA clones from full-length and normalized libraries for gene discovery and functional characterization in citrus.

Authors:  M Carmen Marques; Hugo Alonso-Cantabrana; Javier Forment; Raquel Arribas; Santiago Alamar; Vicente Conejero; Miguel A Perez-Amador
Journal:  BMC Genomics       Date:  2009-09-11       Impact factor: 3.969

4.  Quantitative telomeric overhang determination using a double-strand specific nuclease.

Authors:  Yong Zhao; Hirotoshi Hoshiyama; Jerry W Shay; Woodring E Wright
Journal:  Nucleic Acids Res       Date:  2007-12-10       Impact factor: 16.971
  4 in total

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