Literature DB >> 16732618

A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis.

Wei Wang1, Rita Vignani, Monica Scali, Mauro Cresti.   

Abstract

A simple and universally applicable protocol for extracting high-quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used for routine protein extraction from recalcitrant plant tissues for proteomic analysis.

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Year:  2006        PMID： 16732618     DOI： 10.1002/elps.200500722

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  115 in total

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