BACKGROUND: Myocardial infarction (MI) elicits nerve sprouting. OBJECTIVES: The purpose of this study was to determine the spatial distribution of nerve sprouting and neurotrophic gene expression after MI. METHODS: We created MI in mice by coronary artery ligation. The hearts were removed 3 hours to 2 months after MI and examined for nerve fiber density and neurotrophic factor gene expression using Affymetrix microarray and mRNA analyses. RESULTS: The density of nerve fibers immunopositive for growth-associated protein (GAP)-43 was the highest 3 hours after MI both in the peri-infarct area and in the area remote to infarct, resulting in sympathetic (but not parasympathetic) hyperinnervation in the ventricles. The GAP-43-positive nerve fiber density of myocardium was greater in the outer transverse loop than in the inner vertical loop. The differences between these two myocardial loops peaked within 3 hours after MI and persisted for 2 months afterward. Gene expression of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-beta(3), and interleukin-1alpha was increased up to 2 months after MI compared with normal control. Expression of these growth factors was more pronounced and persistent in the peri-infarct area than in the remote area. CONCLUSION: MI induces sympathetic nerve sprouting in both peri-infarct and remote areas, more in the outer transverse loop. Selective up-regulation of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-beta(3), and interleukin-1alpha occurred in the peri-infarct area and, to a lesser extent, in the remote area.
BACKGROUND:Myocardial infarction (MI) elicits nerve sprouting. OBJECTIVES: The purpose of this study was to determine the spatial distribution of nerve sprouting and neurotrophic gene expression after MI. METHODS: We created MI in mice by coronary artery ligation. The hearts were removed 3 hours to 2 months after MI and examined for nerve fiber density and neurotrophic factor gene expression using Affymetrix microarray and mRNA analyses. RESULTS: The density of nerve fibers immunopositive for growth-associated protein (GAP)-43 was the highest 3 hours after MI both in the peri-infarct area and in the area remote to infarct, resulting in sympathetic (but not parasympathetic) hyperinnervation in the ventricles. The GAP-43-positive nerve fiber density of myocardium was greater in the outer transverse loop than in the inner vertical loop. The differences between these two myocardial loops peaked within 3 hours after MI and persisted for 2 months afterward. Gene expression of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-beta(3), and interleukin-1alpha was increased up to 2 months after MI compared with normal control. Expression of these growth factors was more pronounced and persistent in the peri-infarct area than in the remote area. CONCLUSION: MI induces sympathetic nerve sprouting in both peri-infarct and remote areas, more in the outer transverse loop. Selective up-regulation of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-beta(3), and interleukin-1alpha occurred in the peri-infarct area and, to a lesser extent, in the remote area.
Authors: Stanley F Fernandez; Vladislav Ovchinnikov; John M Canty; James A Fallavollita Journal: Am J Physiol Heart Circ Physiol Date: 2012-11-02 Impact factor: 4.733
Authors: Christina U Lorentz; Diana C Parrish; Eric N Alston; Michael J Pellegrino; William R Woodward; Barbara L Hempstead; Beth A Habecker Journal: Exp Neurol Date: 2013-09-03 Impact factor: 5.330
Authors: Rudolf A Werner; Yoshifumi Maya; Christoph Rischpler; Mehrbod S Javadi; Kazuhito Fukushima; Constantin Lapa; Ken Herrmann; Takahiro Higuchi Journal: Eur J Nucl Med Mol Imaging Date: 2015-08-21 Impact factor: 9.236
Authors: Johanna Lähteenvuo; Olli-Pekka Hätinen; Antti Kuivanen; Jenni Huusko; Jussi Paananen; Markku Lähteenvuo; Jussi Nurro; Marja Hedman; Juha Hartikainen; Nihay Laham-Karam; Petri Mäkinen; Markus Räsänen; Kari Alitalo; Anthony Rosenzweig; Seppo Ylä-Herttuala Journal: Mol Ther Date: 2020-03-19 Impact factor: 11.454