| Literature DB >> 16730477 |
Clairce L S Pagadigorria1, Fernanda Marcon, Ana M Kelmer-Bracht, Adelar Bracht, Emy L Ishii-Iwamoto.
Abstract
The metabolic effects of methotrexate in perfused livers are similar to those exerted by hormones acting through Ca(2+)-dependent mechanisms. The aim of the present study was to determine whether the effects of methotrexate are mediated by a direct action on cellular Ca(2+) fluxes. Methotrexate did not affect the ATP-dependent (45)Ca(2+) uptake by mitochondria, microsomes and inside-out plasma membrane vesicles and Ca(2+) efflux from plasma membrane vesicles. However, methotrexate was able to stimulate (45)Ca(2+) release from preloaded microsomes. The amount of Ca(2+) released by methotrexate was similar to that induced by IP(3). Methotrexate could be acting through the capacitative calcium entry mechanism.Entities:
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Year: 2006 PMID： 16730477 DOI： 10.1016/j.cbpc.2006.03.007
Source DB: PubMed Journal: Comp Biochem Physiol C Toxicol Pharmacol ISSN： 1532-0456 Impact factor: 3.228