Comparison of pregnancy rates achieved with frozen semen using two packaging methods.

The pregnancy rate achieved with frozen semen using two packaging methods was compared in two separate experiments. The sperm-rich fraction of the semen was collected and immediately extended in an egg-yolk based extender to a final concentration of 100 x 10(6) sperm/ml. Half of each ejaculate was packaged in 4.0-ml plastic straws and the other half in 10-to 12-ml flattened aluminum tubes. After detection of a 35-mm follicle, mares were inseminated daily until ovulation. Pregnancy was detected, and embryonic vesicle size was measured by ultrasonic echography Days 14 to 26 after ovulation. The one-cycle pregnancy rate achieved using both packaging methods was 50% overall (23 46 ), with 46% (12 26 ) and 55% (11 20 ) rates achieved, respectively, using straws and aluminum tubes. These rates were not significantly different.